Microbiological characterization and effect of resin composites in cervical lesions.

BACKGROUND

Non carious cervical lesions associated to muscle hyperfunctions are increasing. Microhybrid resin composites are used to restore cervical abfractions. The purpose of this study was to investigate if resin composites modify tooth plaque, inducing an increment of cariogenic microflora and evaluate their effect, and , against .

MATERIAL AND METHODS

Eight abfractions were restored with two microhybrid resin composites (Venus, Heraeus-Kulzer® and Esthet-X, Dentsply®), after gnatological therapy, in three patients with muscle hyperfunctions. For each abfraction three samples of plaque were taken from the cervical perimeter: before the restoration, one week and three months after restoration. The samples were evaluated both by traditional microbiological methods and by Polymerase Chain Reaction (PCR). , disk-shaped specimens of the two composites were prepared to estimate the effects against pre-cultured , after incubation at 37°C for 24h and assessed by a turbidimetric technique.

RESULTS

no differences were found in plaque growth, for all samples, before and after restoration with both composites; , instead, a significant reduction of growth was found between specimens of two composites (Mann-Whitney U-test >0,06).

CONCLUSIONS

In this study a relevant consideration was elicited: composite materials, , do not modify plaque composition of non carious cervical lesions to a potential cariogenic plaque. Abfraction, restoration, S. mutans, composite, class V.