Sharma Ravi Datta, Bogaerts Bert, Goyal Neha
Centre for Microbial and Plant Genetics, KU Leuven, 3000, Belgium; Amity institute of Biotechnology and Amity institute of Integrative Sciences and Health, Amity University Haryana, NH-8, Panchgaon, Gurgaon 122413, India.
Centre for Microbial and Plant Genetics, KU Leuven, 3000, Belgium.
Gene. 2017 Apr 20;609:62-67. doi: 10.1016/j.gene.2017.01.027. Epub 2017 Jan 31.
The splicing factors RDM16 and STA1 have been reported to play a role in the RNA directed DNA Methylation (RdDM) pathway. In this pathway, small interfering RNAs guide de-novo methylation of homologous DNA sequences. DNA methylation is epigenetic marks, which can suppress transposable elements, repeat sequences and genes. It also affects the chromatin structure. Upon deletion of RDM16 and STA1, previous studies based on gene level analysis were unable to find differentially spliced events implicated in RdDM pathway. In this study, RNA-seq data from RDM16 &STA1 mutants were analyzed. Differential expression analysis was performed at exon and intron level. This analysis revealed 3474 genes with potential differential expression events in the RDM16 mutant and 3058 in the STA1 mutant. We found 17 genes that have been reported to be involved in the RdDM pathway. These results suggest involvement of RDM16 and STA1 by influencing the expression of key gene components such as MORC6 in the RdDM pathway. Therefore, this study increases the understanding of the role of RDM16 and STA1 splicing factors in DNA methylation.
据报道，剪接因子RDM16和STA1在RNA指导的DNA甲基化（RdDM）途径中发挥作用。在该途径中，小干扰RNA指导同源DNA序列的从头甲基化。DNA甲基化是一种表观遗传标记，可抑制转座元件、重复序列和基因。它还会影响染色质结构。在缺失RDM16和STA1后，先前基于基因水平分析的研究未能发现与RdDM途径相关的差异剪接事件。在本研究中，对来自RDM16和STA1突变体的RNA-seq数据进行了分析。在外显子和内含子水平上进行了差异表达分析。该分析揭示了RDM16突变体中3474个具有潜在差异表达事件的基因，以及STA1突变体中3058个。我们发现了17个据报道参与RdDM途径的基因。这些结果表明，RDM16和STA1通过影响RdDM途径中关键基因成分（如MORC6）的表达而发挥作用。因此，本研究增进了对RDM16和STA1剪接因子在DNA甲基化中作用的理解。
Zotero 插件