Sirotkin Alexander V, Florkovičová Koničková Iveta, Schaeffer Hans-Jorg, Laurincik Jozef, Harrath Abdel Halim
Dept. Zoology and Anthropology, Constantine the Philosopher University, 949 74 Nitra, Slovakia; Dept. Genetics and Reproduction, Research Institute of Animal Production, 949 59 Lužianky, Slovakia.
Dept. Genetics and Reproduction, Research Institute of Animal Production, 949 59 Lužianky, Slovakia.
Reprod Biol. 2017 Mar;17(1):97-104. doi: 10.1016/j.repbio.2017.01.005. Epub 2017 Feb 2.
Dominance or cooperation between ovarian follicles can determine the number of ovulations and fecundity, but interrelationships between follicles in mono- and poly-ovulatory species and their mechanisms are poorly understood. The goals of this work were to determine the existence and compare the character of mutual influence of cultured ovarian follicles from a mono-ovulatory species (cow) with established follicular dominance with those from a poly-ovulatory species (pig), in which interrelationship between follicles remain unknown, and to examine the role of ovarian cell proliferation, the insulin-like growth factor I (IGF-I)- oxytocin (OT) system, and steroid hormones in mediating interrelationships among ovarian follicles. Bovine and porcine ovarian follicles were isolated and cultured alone and in pairs, and the percentage of growing follicles was calculated. Porcine follicles were cultured alone and in pairs after addition of exogenous OT and IGF-I (100ngmL) or inactivation of endogenous OT and IGF-I by antisera against these hormones (1%). Proliferation of porcine follicular cells was assessed by SDS PAGE-Western immunoblotting, the release of IGF-I, progesterone, androstenedione and estradiol by cultured porcine ovarian follicles was analyzed by RIA/EIA. Overall, our observations suggest (1) competition/dominance (mutual suppression of growth) in bovine ovarian follicles, (2) cooperation (mutual support of growth) in porcine ovarian follicles, (3) that this mutual growth of porcine ovarian follicles was caused by the promotion of cell proliferation, (4) that this mechanism was probably not involved in bovine follicular dominance, (5) that communication between both porcine and bovine follicles affects their secretory activity, and (6) that both follicular dominance in cows and cooperation of follicles in pigs can be mediated by either down- or up-regulation of the IGF-I-OT system, which in turn affects follicular steroidogenesis and promotes follicular cell proliferation and follicular growth.
卵巢卵泡之间的优势或协同作用可决定排卵数量和生育力，但单排卵和多排卵物种中卵泡之间的相互关系及其机制尚不清楚。本研究的目的是确定单排卵物种（母牛）已建立卵泡优势的培养卵巢卵泡与多排卵物种（猪）的培养卵巢卵泡之间相互影响的存在情况并比较其特征，猪卵泡之间的相互关系尚不清楚，同时研究卵巢细胞增殖、胰岛素样生长因子I（IGF-I）-催产素（OT）系统和类固醇激素在介导卵巢卵泡之间相互关系中的作用。将牛和猪的卵巢卵泡单独或成对分离培养，并计算生长卵泡的百分比。在添加外源性OT和IGF-I（100ng/mL）或用针对这些激素的抗血清（1%）使内源性OT和IGF-I失活后，将猪卵泡单独或成对培养。通过SDS-PAGE- Western免疫印迹法评估猪卵泡细胞的增殖，通过放射免疫分析/酶免疫分析（RIA/EIA）分析培养的猪卵巢卵泡释放的IGF-I、孕酮、雄烯二酮和雌二醇。总体而言，我们的观察结果表明：（1）牛卵巢卵泡存在竞争/优势（生长的相互抑制）；（2）猪卵巢卵泡存在协同作用（生长的相互支持）；（3）猪卵巢卵泡的这种共同生长是由细胞增殖的促进引起的；（4）该机制可能不参与牛卵泡优势的形成；（5）猪和牛卵泡之间的通讯均影响其分泌活性；（6）母牛的卵泡优势和猪卵泡的协同作用均可通过IGF-I-OT系统的下调或上调介导，进而影响卵泡类固醇生成并促进卵泡细胞增殖和卵泡生长。
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