通过[具体物种1]和[具体物种2]之间的属间杂交培育抗[具体对象]的植物。

Development of plants resistant to by intergeneric hybridization between and .

作者信息

Tarora Kazuhiko, Shudo Ayano, Kawano Shinji, Yasuda Keiji, Ueno Hiroki, Matsumura Hideo, Urasaki Naoya

机构信息

Okinawa Prefectural Agricultural Research Center, 820 Makabe, Itoman, Okinawa 901-0336, Japan; Department of Bioscience and Textile Technology, Shinshu University, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan.

Okinawa Prefectural Agricultural Research Center , 820 Makabe, Itoman, Okinawa 901-0336 , Japan.

出版信息

Breed Sci. 2016 Dec;66(5):734-741. doi: 10.1270/jsbbs.16107. Epub 2016 Oct 29.

DOI:10.1270/jsbbs.16107

PMID:28163589

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5282761/

Abstract

In this study, we confirmed that resists (PLDMV), and used it to produce intergeneric hybrids with . From the cross between and , we obtained 147 seeds with embryos. Though is a monoembryonic plant, multiple embryos were observed in all 147 seeds. We produced 218 plants from 28 seeds by means of embryo-rescue culture. All plants had pubescence on their petioles and stems characteristic of . Flow cytometry and PCR of 28 plants confirmed they were intergeneric hybrids. To evaluate virus resistance, mechanical inoculation of PLDMV was carried out. The test showed that 41 of 134 intergeneric hybrid plants showed no symptoms and were resistant. The remaining 93 hybrids showed necrotic lesions on the younger leaves than the inoculated leaves. In most of the 93 hybrids, the necrotic lesions enclosed the virus and prevented further spread. These results suggest that the intergeneric hybrids will be valuable material for PLDMV-resistant papaya breeding.

摘要

在本研究中，我们确认了抗性（番木瓜叶畸形病毒），并利用它与……培育属间杂种。通过……与……的杂交，我们获得了147粒带有胚的种子。尽管……是单胚植物，但在所有147粒种子中均观察到多个胚。我们通过胚挽救培养从28粒种子培育出218株植株。所有植株的叶柄和茎上都有……特有的柔毛。对28株植株进行的流式细胞术和PCR检测证实它们是属间杂种。为评估病毒抗性，对番木瓜叶畸形病毒进行了机械接种。试验表明，134株属间杂种植物中有41株未出现症状且具有抗性。其余93株杂种在比接种叶更嫩的叶片上出现坏死斑。在93株杂种中的大多数中，坏死斑包围了病毒并阻止其进一步传播。这些结果表明，属间杂种将成为抗番木瓜叶畸形病毒木瓜育种的宝贵材料。