与肺炎球菌岩藻糖利用相关的磷酸转移酶系统IIA和IIB蛋白的结构表征。

Structural characterization of the PTS IIA and IIB proteins associated with pneumococcal fucose utilization.

作者信息

Higgins Melanie A, Hamilton Aileen M, Boraston Alisdair B

机构信息

Biochemistry and Microbiology, University of Victoria, PO Box 3055 STN CSC, Victoria, British Columbia, V8W 3P6, Canada.

出版信息

Proteins. 2017 May;85(5):963-968. doi: 10.1002/prot.25264. Epub 2017 Mar 24.

DOI:10.1002/prot.25264

PMID:28168775

Abstract

Streptococcus pneumoniae harbors a significant number of transporters, including phosphotransferase (PTS) systems, allowing the bacterium to utilize a number of different carbohydrates for metabolic and other purposes. The genes encoding for one PTS transport system in particular (EII ) are found within a fucose utilization operon in S. pneumoniae TIGR4. Here, we report the three-dimensional structures of IIA and IIB providing evidence that this PTS system belongs to the EII family. Additionally, the predicted metabolic pathway for this distinctive fucose utilization system suggests that EII transports the H-disaccharide blood group antigen, which would represent a novel PTS transporter specificity. Proteins 2017; 85:963-968. © 2016 Wiley Periodicals, Inc.

摘要

肺炎链球菌含有大量转运蛋白，包括磷酸转移酶（PTS）系统，这使得该细菌能够利用多种不同的碳水化合物用于代谢及其他目的。编码一种特定PTS转运系统（EII）的基因尤其存在于肺炎链球菌TIGR4的一个岩藻糖利用操纵子内。在此，我们报道了IIA和IIB的三维结构，提供了该PTS系统属于EII家族的证据。此外，这种独特的岩藻糖利用系统的预测代谢途径表明，EII转运H-二糖血型抗原，这将代表一种新型的PTS转运蛋白特异性。《蛋白质》2017年；85卷：963 - 968页。©2016威利期刊公司。