Li Yang, Sheng Ying, Liang JianMin, Ren XiaoYong, Cheng Yan
Departments of aOtolaryngology-Head and Neck Surgery bDigestive Diseases, The Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
Neuroreport. 2017 Mar 22;28(5):235-241. doi: 10.1097/WNR.0000000000000731.
Schwann cell (SC) transplantation as a cell-based therapy can enhance peripheral and central nerve repair experimentally, but it is limited by donor site morbidity for clinical application. We investigated whether human turbinate-derived mesenchymal stem cells (hTMSCs) isolated from discarded inferior turbinate during surgery can differentiate into functional SC-like cells. hTMSCs expressed mesenchymal cell surface markers CD29, CD44, CD90, and CD105 and did not express neural crest markers P75 and Nestin. After monolayer culture in predifferentiation medium and transdifferentiation medium with a mixture of glial growth factors and chemical regents for 14 days, the differentiated hTMSCs exhibited a spindle-like morphology similar to that of SCs. RT-PCR, immunocytochemical staining, and western blotting analysis indicated that SC-like cells expressed the glial markers S100β, P75, and glial fibrillary acidic protein at the gene and protein level. Compared with hTMSCs, differentiated hTMSCs secreted more neurotrophins, and significantly enhanced the neurite length when cocultured with dorsal root ganglia neuronal cells. Our data indicated that hTMSCs can differentiate into functional SC-like cells and have the ability to facilitate the neurite growth of dorsal root ganglia neuronal cells in vitro, representing a promising source of cells for nerve repair.
雪旺细胞（SC）移植作为一种基于细胞的治疗方法，在实验中可促进周围神经和中枢神经的修复，但临床应用中受供体部位发病率的限制。我们研究了从手术中废弃的下鼻甲分离出的人鼻甲间充质干细胞（hTMSC）是否能分化为功能性雪旺细胞样细胞。hTMSC表达间充质细胞表面标志物CD29、CD44、CD90和CD105，不表达神经嵴标志物P75和巢蛋白。在含有胶质生长因子和化学试剂混合物的预分化培养基及转分化培养基中进行单层培养14天后，分化的hTMSC呈现出与雪旺细胞相似的纺锤样形态。逆转录聚合酶链反应（RT-PCR）、免疫细胞化学染色和蛋白质印迹分析表明，雪旺细胞样细胞在基因和蛋白质水平上表达胶质标志物S100β、P75和胶质纤维酸性蛋白。与hTMSC相比，分化的hTMSC分泌更多的神经营养因子，与背根神经节神经元细胞共培养时显著增加了神经突长度。我们的数据表明，hTMSC可分化为功能性雪旺细胞样细胞，并在体外具有促进背根神经节神经元细胞神经突生长的能力，是一种很有前景的神经修复细胞来源。
