虹鳟（Salmo gairdneri）卵巢液中富含脱氨神经氨酸糖蛋白（KDN-gp-OF）的分离与鉴定。
Isolation and characterization of deaminated neuraminic acid-rich glycoprotein (KDN-gp-OF) in the ovarian fluid of rainbow trout (Salmo gairdneri).
作者信息
Kanamori A, Kitajima K, Inoue S, Inoue Y
机构信息
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
出版信息
Biochem Biophys Res Commun. 1989 Oct 31;164(2):744-9. doi: 10.1016/0006-291x(89)91522-2.
A novel highly acidic glycoprotein (deaminated neuraminic acid-rich glycoprotein; KDN-gp) was first discovered as an integral component of the vitelline envelope of rainbow trout eggs [Inoue, S., et al. (1988) Biochem. Biophys. Res. Commun. 153, 172-176]. Another member of this class of glycoprotein has now been found in the ovarian (or coelomic) fluid of ovulating rainbow trout. This ovarian fluid KDN-glycoprotein is designated as KDN-gp-OF and its amino acid and carbohydrate compositions were compared with those of the vitelline envelope KDN-gp (KDN-gp-VE). KDN-gp-OF was similar to KDN-gp-VE in the carbohydrate composition and molecular weight. However, a small but definite difference in amino acid composition and the molecular weight range was found between KDN-gp-OF and KDN-gp-VE. The results suggest that in KDN-gp-OF some peptide sequences presumably present at either the C- or N-terminus are deleted from KDN-gp-VE. Possible biological function of KDN-gp-OF is discussed.
一种新型的高酸性糖蛋白（富含脱氨基神经氨酸的糖蛋白；KDN - gp）最初被发现是虹鳟鱼卵黄膜的一个组成成分[井上，S.等人（1988年）《生物化学与生物物理研究通讯》153，172 - 176]。现在在排卵的虹鳟鱼的卵巢（或体腔）液中发现了这类糖蛋白的另一个成员。这种卵巢液KDN - 糖蛋白被命名为KDN - gp - OF，并将其氨基酸和碳水化合物组成与卵黄膜KDN - gp（KDN - gp - VE）的进行了比较。KDN - gp - OF在碳水化合物组成和分子量方面与KDN - gp - VE相似。然而，在KDN - gp - OF和KDN - gp - VE之间发现了氨基酸组成和分子量范围上的一个小但确定的差异。结果表明，在KDN - gp - OF中，可能存在于C端或N端的一些肽序列从KDN - gp - VE中缺失了。文中讨论了KDN - gp - OF可能的生物学功能。
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