Takagui Fábio Hiroshi, Moura Lucas Ferreira de, Ferreira Daniela Cristina, Centofante Liano, Vitorino Carla de Andrade, Bueno Vanessa, Margarido Vladimir Pavan, Venere Paulo Cesar
1 Laboratório de Citogenética Animal, Departamento de Biologia Geral, CCB, Universidade Estadual de Londrina , Londrina, Brazil .
2 Laboratório de Ictiologia, Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso , Pontal do Araguaia, Brazil .
Zebrafish. 2017 Jun;14(3):236-243. doi: 10.1089/zeb.2016.1368. Epub 2017 Feb 13.
Karyotypes and other chromosomal markers as revealed by conventional and molecular cytogenetic protocols in four species of the catfish family Doradidae from the Araguaia-Tocantins river basin, namely Hassar wilderi, Leptodoras cataniae, Tenellus leporhinus and Tenellus trimaculatus were examined. All species had diploid chromosome number 2n = 58 and karyotypes dominated by biarmed chromosomes, simple NOR phenotype, that is, one chromosome pair bearing this site in terminal position, but some differences in karyotypes and distribution of constitutive heterochromatin, position of rDNA sites. Such characteristics appeared species-specific. A ZZ/ZW sex chromosome system was found in Tenellus trimaculatus, resulting likely from the amplification of the heterochromatin, followed by a paracentric inversion. Our results confirmed low karyotype differentiation observed until now among representatives of this endemic catfish family.
对来自阿拉瓜亚 - 托坎廷斯河流域的鲶鱼科多拉多鲶属四个物种，即哈氏野鲶、卡氏细体鲶、兔鼻小鲶和三斑小鲶，通过传统和分子细胞遗传学方法揭示的核型及其他染色体标记进行了研究。所有物种的二倍体染色体数均为2n = 58，核型以双臂染色体为主，具有简单的核仁组织区（NOR）表型，即一对染色体在末端位置带有该位点，但在核型、组成型异染色质分布、核糖体DNA（rDNA）位点位置上存在一些差异。这些特征具有物种特异性。在三斑小鲶中发现了ZZ/ZW性染色体系统，这可能是由于异染色质扩增，随后发生了臂间倒位导致的。我们的结果证实了迄今为止在这个特有鲶鱼科代表物种中观察到的低核型分化。
