基于疏水碳点的双发射胶束用于肝细胞中 Cu 的比率荧光生物传感和成像。
Hydrophobic-carbon-dot-based dual-emission micelle for ratiometric fluorescence biosensing and imaging of Cu in liver cells.
机构信息
School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.
Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China.
出版信息
Biosens Bioelectron. 2017 Jun 15;92:101-108. doi: 10.1016/j.bios.2017.01.066. Epub 2017 Jan 31.
Copper is closely related to liver damage, therefore, it is essential to develop a simple and sensitive strategy to detect copper ions (Cu) in liver cells. A hydrophobic carbon dots (HCDs)-based dual-emission fluorescent probe for Cu was prepared by encapsulating HCDs in micelles formed by self-assembly of amphiphilic polymer DSPE-PEG and tetrakis (4-carboxyphenyl) porphyrin (TCPP)-modified DSPE-PEG. The obtained probe showed characteristic fluorescence emissions of HCDs and TCPP with large emission shift of 170nm with single-wavelength excitation. In the presence of Cu, the fluorescence of TCPP was quenched and that of HCDs remained unchanged, displaying ratiometric fluorescence response to Cu. The developed probe exhibited high sensitivity (detection limit down to 36nM) and selectivity to Cu over other substances, and the probe was used to image the changes of Cu level in liver cells successfully.
铜与肝损伤密切相关，因此，开发一种简单、灵敏的方法来检测肝细胞中的铜离子（Cu）至关重要。通过将碳点（HCDs）封装在由两亲聚合物 DSPE-PEG 和四（4-羧基苯基）卟啉（TCPP）改性的 DSPE-PEG 自组装形成的胶束中，制备了基于疏水性碳点（HCDs）的双发射荧光探针用于 Cu。所得探针表现出 HCDs 和 TCPP 的特征荧光发射，单波长激发时有 170nm 的大发射位移。在 Cu 的存在下，TCPP 的荧光被猝灭，而 HCDs 的荧光保持不变，对 Cu 表现出比率荧光响应。该探针对 Cu 具有高灵敏度（检测限低至 36nM）和选择性，并且该探针成功用于成像肝细胞中 Cu 水平的变化。
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