Tn602：一种天然存在的Tn903的相关物，带有同向重复序列。

Tn602: a naturally occurring relative of Tn903 with direct repeats.

作者信息

Stibitz S, Davies J E

出版信息

Plasmid. 1987 May;17(3):202-9. doi: 10.1016/0147-619x(87)90028-x.

DOI:10.1016/0147-619x(87)90028-x

Abstract

We report the characterization of Tn602, a transposon encoding resistance to kanamycin and related aminoglycosides present on the R-plasmid pGD10. Tn602 is highly homologous to the previously characterized Tn903, present on the R-plasmid R6, in that it consists of a gene for aminoglycoside-phosphotransferase-3'-I (homologous to that of Tn903) flanked by copies of an IS-element homologous to IS903. Tn602 differs from Tn903 in the following respects: the flanking IS-elements (IS602) are in direct rather than inverted orientation as in Tn903; the fusion points between the IS-elements and the central region are different from those in Tn903; and several sequence changes, detected by the loss and acquisition of restriction sites, show the two repeats of IS602 to be nonidentical and different from IS903, IS102, and IS903.B. These structural details suggest that Tn602 and Tn903 evolved separately from related modules.

摘要

我们报道了转座子Tn602的特性，它编码对卡那霉素及相关氨基糖苷类抗生素的抗性，存在于R质粒pGD10上。Tn602与先前鉴定的存在于R质粒R6上的Tn903高度同源，因为它由一个氨基糖苷磷酸转移酶-3'-I基因（与Tn903的该基因同源）组成，两侧是与IS903同源的IS元件拷贝。Tn602在以下方面与Tn903不同：侧翼的IS元件（IS602）呈直接排列，而不像Tn903那样呈反向排列；IS元件与中心区域之间的融合点与Tn903中的不同；通过限制酶切位点的缺失和获得检测到的几个序列变化表明，IS602的两个重复序列不相同，且与IS903、IS102和IS903.B不同。这些结构细节表明Tn602和Tn903是从相关模块分别进化而来的。