Wang Biao, Russell Margaret L, Fonseca Kevin, Earn David J D, Horsman Gregory, Van Caeseele Paul, Chokani Khami, Vooght Mark, Babiuk Lorne, Walter Stephen D, Loeb Mark
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
Department of Community Health Sciences, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Influenza Other Respir Viruses. 2017 May;11(3):254-262. doi: 10.1111/irv.12448. Epub 2017 Mar 16.
Patterns of influenza molecular viral shedding following influenza infection have been well established; predictors of viral shedding however remain uncertain.
We sought to determine factors associated with peak molecular viral load, duration of shedding, and viral area under the curve (AUC) in children and adult Hutterite colony members with laboratory-confirmed influenza.
A cohort study was conducted in Hutterite colonies in Alberta, Canada. Flocked nasal swabs were collected during three influenza seasons (2007-2008 to 2009-2010) from both symptomatic and asymptomatic individuals infected with influenza. Samples were tested by real-time reverse-transcription polymerase chain reaction for influenza A and influenza B, and the viral load was determined for influenza A-positive samples.
For seasonal H1N1, younger age was associated with a larger AUC, female sex was associated with decreased peak viral load and reduced viral shedding duration, while the presence of comorbidity was associated with increased peak viral load. For H3N2, younger age was associated with increased peak viral load and increased AUC. For pandemic H1N1, younger age was associated with increased peak viral load and increased viral AUC, female sex was associated with reduced peak viral load, while inapparent infection was associated with reduced peak viral load, reduced viral shedding duration, and reduced viral AUC.
Patterns of molecular viral shedding vary by age, sex, comorbidity, and the presence of symptoms. Predictor variables vary by influenza A subtype.
流感感染后流感病毒分子脱落模式已得到充分证实；然而，病毒脱落的预测因素仍不确定。
我们试图确定实验室确诊流感的儿童和成年哈特人殖民地成员中，与分子病毒载量峰值、病毒脱落持续时间和病毒曲线下面积（AUC）相关的因素。
在加拿大艾伯塔省的哈特人殖民地进行了一项队列研究。在三个流感季节（2007 - 2008年至2009 - 2010年）期间，从有症状和无症状的流感感染者中采集植绒鼻拭子。通过实时逆转录聚合酶链反应检测甲型和乙型流感样本，并对甲型流感阳性样本测定病毒载量。
对于季节性H1N1，年龄较小与较大的AUC相关，女性与峰值病毒载量降低和病毒脱落持续时间缩短相关，而合并症的存在与峰值病毒载量增加相关。对于H3N2，年龄较小与峰值病毒载量增加和AUC增加相关。对于大流行性H1N1，年龄较小与峰值病毒载量增加和病毒AUC增加相关，女性与峰值病毒载量降低相关，而隐性感染与峰值病毒载量降低、病毒脱落持续时间缩短和病毒AUC降低相关。
分子病毒脱落模式因年龄、性别、合并症和症状的存在而有所不同。预测变量因甲型流感亚型而异。
