Elghanam Ghada Abu, Liu Younan, Khalili Saeed, Fang Dongdong, Tran Simon D
Craniofacial Tissue Engineering and Stem Cells Laboratory, Faculty of Dentistry, McGill University, 3640 University Street, M43, Montreal, QC, Canada.
University of Jordan, Amman, Jordan.
Methods Mol Biol. 2017;1553:25-39. doi: 10.1007/978-1-4939-6756-8_3.
Compact bone (cortical or dense bone) is among the organs that contain multipotent mesenchymal stromal cells (MSCs). Unlike bone marrow plugs where MSCs were initially isolated, compact bone has minimal (amount of) hematopoietic cells and thus facilitates the MSCs isolation process. In vitro, MSCs from compact bone show multipotency and differentiation into mesenchymal tissues such as bone, adipose, and cartilage, under certain conditions. MSCs therapy has been promising in preclinical and clinical studies against autoimmune diseases. Not only can MSCs replace the lost tissue through their regenerative properties, but they can also control the autoimmune attacks by immunoregulatory cytokines. This protocol describes the use of compact bone-derived MSCs to preserve salivary function (saliva flow/output) in the NOD (nonobese diabetic) mouse model affected with Sjogren's-like disease.
密质骨（皮质骨或致密骨）是含有多能间充质基质细胞（MSC）的器官之一。与最初分离出MSC的骨髓栓不同，密质骨中的造血细胞极少，因此便于MSC的分离过程。在体外，来自密质骨的MSC在某些条件下表现出多能性，并能分化为间充质组织，如骨、脂肪和软骨。在针对自身免疫性疾病的临床前和临床研究中，MSC治疗前景广阔。MSC不仅可以通过其再生特性替代受损组织，还能通过免疫调节细胞因子控制自身免疫攻击。本方案描述了在患有类干燥综合征的非肥胖糖尿病（NOD）小鼠模型中，使用源自密质骨的MSC来维持唾液功能（唾液流量/分泌量）的方法。
