Chen Jiazhang, Meng Shunlong, Xu Hai, Zhang Zhen, Wu Xiangyang
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
Key Laboratory of Fishery Eco-environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze River, CAFS, Wuxi, 214081, China.
Bull Environ Contam Toxicol. 2017 Apr;98(4):562-566. doi: 10.1007/s00128-017-2051-4. Epub 2017 Feb 24.
In the present study, Nile tilapia (Oreochromis niloticus) were used to assess the endocrine disruption potential of Microcytis aeruginosa. Male Nile tilapia were exposed to lyophilized M. aeruginosa or purified microcystin-LR (8.3 μg/L) for 28 days. The levels of serum hormones (17β-estradiol and testosterone) and transcripts of selected genes in the hypothalamus-pituitary-gonadal-liver axis were analyzed. The results showed that serum hormones were significantly up-regulated, and transcripts of 13 genes (GHRH, PACAP, GH, GHR1, GHR2, IGF1, IGF2, CYP19a, CYP19b, 3β-HSD1, 20β-HSD, 17β-HSD1 and 17β-HSD8) were significantly altered after Microcytis exposure. These results indicate that fish reproduction can be altered in a Microcystis bloom-contaminated aquatic environment.
在本研究中，尼罗罗非鱼（Oreochromis niloticus）被用于评估铜绿微囊藻的内分泌干扰潜力。雄性尼罗罗非鱼暴露于冻干的铜绿微囊藻或纯化的微囊藻毒素-LR（8.3μg/L）中28天。分析了血清激素（17β-雌二醇和睾酮）水平以及下丘脑-垂体-性腺-肝脏轴中选定基因的转录本。结果表明，暴露于铜绿微囊藻后，血清激素显著上调，13个基因（生长激素释放激素、垂体腺苷酸环化酶激活肽、生长激素、生长激素受体1、生长激素受体2、胰岛素样生长因子1、胰岛素样生长因子2、细胞色素P450 19a、细胞色素P450 19b、3β-羟基类固醇脱氢酶1、20β-羟基类固醇脱氢酶、17β-羟基类固醇脱氢酶1和17β-羟基类固醇脱氢酶8）的转录本也发生了显著变化。这些结果表明，在受微囊藻水华污染的水生环境中，鱼类繁殖可能会受到影响。
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