Bang Jeongsu, Li Ling, Seong Hyunbin, Kwon Ye Won, Lee Dong-Yup, Han Nam Soo
Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea.
Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Farm Produces, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, P.R. China.
J Microbiol Biotechnol. 2017 May 28;27(5):939-942. doi: 10.4014/jmb.1612.12038.
The cellular composition and metabolic compounds of ATCC 8293 were analyzed after cultivation in an anaerobic chemostat. The macromolecular composition was 24.4% polysaccharide, 29.7% protein, 7.9% lipid, 2.9% DNA, and 7.4% RNA. Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine. Elements were in the order of C > O > N > H > S. The metabolites in chemostat culture were lactic acid (73.34 mM), acetic acid (7.69 mM), and mannitol (9.93 mM). These data provide a first view of the cellular composition of for use in metabolic flux analysis.
在厌氧恒化器中培养后，对ATCC 8293的细胞组成和代谢化合物进行了分析。其大分子组成包括24.4%的多糖、29.7%的蛋白质、7.9%的脂质、2.9%的DNA和7.4%的RNA。其氨基酸组成包括大量的赖氨酸、谷氨酸、丙氨酸和亮氨酸。元素含量顺序为C＞O＞N＞H＞S。恒化器培养中的代谢产物为乳酸（73.34 mM）、乙酸（7.69 mM）和甘露醇（9.93 mM）。这些数据为用于代谢通量分析的细胞组成提供了初步认识。
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