Wu Yan, Guo Shan-Shan, Huang Dong-Ye, Wang Cheng-Fang, Wei Jian-Yu, Li Zhi-Hua, Sun Jian-Sheng, Bai Jia-Feng, Tian Zhao-Fu, Wang Ping-Juan, Du Shu-Shan
Technical Center of China Tobacco Guangxi Industrial Co., Ltd.
J Oleo Sci. 2017 Apr 3;66(4):399-405. doi: 10.5650/jos.ess16166. Epub 2017 Feb 24.
The contact toxicity and repellent activities of the essential oil extracted from the rhizomes of Zingiber zerumbet (L.) Smith (Zingiberaceae) was evaluated against cigarette beetles (Lasioderma serricorne). The essential oil obtained by hydrodistillation was investigated by GC-FID and GC-MS. The main constituents of the essential oil were zerumbone (40.2%), α-caryophyllene (8.6%), humulene epoxide II (7.3%), camphene (5.9%) and fenchene (4.7%). Zerumbone and its analogues totally are accounting for 60.3% of the essential oil. It was found that the essential oil possessed contact toxicity against L. serricorne adults with a LD value of 48.3 µg/adult. α-Caryophyllene (LD = 13.1 µg/adult) exhibited stronger contact toxicity against L. serricorne than humulene oxide (LD = 31.2 µg/adult), β-caryophyllene (LD = 35.5 µg/adult) and zerumbone (LD = 42.4 µg/adult). Moreover, α-caryophyllene possessed strong repellent activity (Class IV and V, respectively) against the beetles at 78.63 nL/cm, after 2 and 4 h treatment. The results indicate that zerumbone and its analogues might be developed into natural insecticides or repellents for control of cigarette beetles, but their bioactivities are affected by their structures.
对从姜科植物红球姜（Zingiber zerumbet (L.) Smith）根茎中提取的挥发油进行了针对烟草甲（Lasioderma serricorne）的触杀毒性和驱避活性评估。采用气相色谱 - 氢火焰离子化检测器（GC - FID）和气相色谱 - 质谱联用仪（GC - MS）对通过水蒸馏法获得的挥发油进行了研究。该挥发油的主要成分是姜酮（40.2%）、α - 石竹烯（8.6%）、氧化律草烯Ⅱ（7.3%）、莰烯（5.9%）和小茴香烯（4.7%）。姜酮及其类似物在挥发油中所占比例总计为60.3%。研究发现，该挥发油对烟草甲成虫具有触杀毒性，致死剂量（LD）值为48.3 μg/成虫。α - 石竹烯（LD = 13.1 μg/成虫）对烟草甲的触杀毒性比氧化律草烯（LD = 31.2 μg/成虫）、β - 石竹烯（LD = 35.5 μg/成虫）和姜酮（LD = 42.4 μg/成虫）更强。此外，在处理2小时和4小时后，α - 石竹烯在78.63 nL/cm时对烟草甲具有很强的驱避活性（分别为Ⅳ级和Ⅴ级）。结果表明，姜酮及其类似物可能被开发成用于防治烟草甲的天然杀虫剂或驱避剂，但其生物活性受其结构影响。
