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蛋白水解修饰对热处理蛋清凝胶质地和咀嚼性的影响。

Effect of Proteolytic Modification on Texture and Mastication of Heat-Treated Egg White Gels.

作者信息

Funaki Junko, Minami Michiko, Abe Sachie, Ueda Reiko, Eto Wakako, Kugino Kenji, Kugino Mutsuko, Abe Keiko, Toko Kiyoshi, Asakura Tomiko

机构信息

International College of Arts and Sciences Fukuoka Women's University 1-1-1 Kasumigaoka Higashi-ku Fukuoka 813-8529 Japan; Graduate School of Human Environment of Sciences Fukuoka Women's University 1-1-1 Kasumigaoka Higashi-kun Fukuoka 813-8529 Japan.

Faculty of Education Tokyo Gakugei University 4-1-1 Nukuikitamachi Koganeishi Tokyo 184-8501 Japan.

出版信息

J Food Process Preserv. 2017 Feb;41(1):e12857. doi: 10.1111/jfpp.12857. Epub 2016 May 6.

Abstract

UNLABELLED

Raw egg white undergoes sol-gel transition by heat treatment, which changes it to an elastic gel. Here, protease treatment to render a new texture to heated egg white gel was applied. Protease-treated gels exhibited ductile flow without obvious rupture points. Transmission electron microscopy analysis showed that in protease-treated gels, protein aggregates were distributed more homogeneously compared with that observed in the untreated control, probably because ovalbumin was digested into small peptides as revealed by SDS-PAGE. The properties of the gel were evaluated by sensory tests and by measuring the movement of the masseter muscle, using surface electromyography. Results showed that maximum bite force and mastication duration were decreased for the protease-treated gels, which were evaluated as being softer, smoother, less elastic and better textured. Overall, our results indicate that protease-treated egg white gel has superior qualities and is easier to swallow than the untreated gel.

PRACTICAL APPLICATIONS

In the food industry, the use of egg white is limited compared with that of egg yolk and whole eggs. In this study, we performed protease treatment to generate a new food material with smoother and softer texture compared with heat treated egg white. Our findings may expand the consumption of egg white, which can be consumed by people with mastication and swallowing disorders, and reduce the waste of egg white as a surplus product.

摘要

未标记

生蛋清通过热处理会发生溶胶-凝胶转变,转变为弹性凝胶。在此,我们对加热后的蛋清凝胶进行蛋白酶处理以赋予其新质地。经蛋白酶处理的凝胶呈现出韧性流动且无明显破裂点。透射电子显微镜分析表明,与未处理的对照相比,经蛋白酶处理的凝胶中蛋白质聚集体分布更均匀,这可能是因为如SDS-PAGE所示,卵清蛋白被消化成了小肽。通过感官测试和使用表面肌电图测量咬肌运动来评估凝胶的特性。结果显示,经蛋白酶处理的凝胶的最大咬合力和咀嚼持续时间降低,其被评估为更软、更光滑、弹性更小且质地更好。总体而言,我们的结果表明,经蛋白酶处理的蛋清凝胶具有优异品质,并且比未处理的凝胶更易于吞咽。

实际应用

在食品工业中,与蛋黄和全蛋相比,蛋清的使用受到限制。在本研究中,我们进行蛋白酶处理以生成一种与热处理蛋清相比质地更光滑、更柔软的新型食品原料。我们的研究结果可能会扩大蛋清的消费量,可供有咀嚼和吞咽障碍的人食用,并减少作为剩余产品的蛋清浪费。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9352/5297981/2b3a8a8e7444/JFPP-41-0-g001.jpg

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