Hansen W E
II. Department of Medicine, Technical University of Munich, F.R.G.
Int J Pancreatol. 1986 Dec;1(5-6):341-51. doi: 10.1007/BF02801866.
Chymotrypsin, trypsin, carboxypeptidase A and B, elastase and enterokinase activities were measured in buffer solutions and in human duodenal juice after incubation with wheat bran, cellulose, guar gum, pectin, psyllium and lignin. The different types of dietary fiber led to inhibition of enzymatic activity in most experiments, e.g., lignin could totally ablish the activity of isolated trypsin and chymotrypsin. Only in enterokinase was there no influence. Inhibition depended on incubation time; the effect was proportional to fiber concentration and inversely related to enzyme level. Treatment of fiber with hydrochloric acid (pH 1.5) and heat (95 degrees C) destroyed inhibitory activity in some experiments. The effect of lignin on one enzyme (trypsin) was reduced by the addition of another enzyme (chymotrypsin). It is concluded that dietary fiber could affect digestion by inhibiting proteolytic pancreatic enzymes.
在缓冲溶液以及与麦麸、纤维素、瓜尔豆胶、果胶、车前草籽壳和木质素孵育后的人十二指肠液中，测定了胰凝乳蛋白酶、胰蛋白酶、羧肽酶A和B、弹性蛋白酶及肠激酶的活性。在大多数实验中，不同类型的膳食纤维会导致酶活性受到抑制，例如，木质素可完全消除分离出的胰蛋白酶和胰凝乳蛋白酶的活性。只有肠激酶不受影响。抑制作用取决于孵育时间；其效果与纤维浓度成正比，与酶水平成反比。在一些实验中，用盐酸（pH 1.5）和加热（95摄氏度）处理纤维可破坏抑制活性。添加另一种酶（胰凝乳蛋白酶）可降低木质素对一种酶（胰蛋白酶）的影响。得出的结论是，膳食纤维可通过抑制胰腺蛋白水解酶来影响消化。
