Vyatkina Kira
Center for Algorithmic Biotechnology, Institute of Translational Biomedicine, Saint Petersburg State University, 7-9 Universitetskaya nab., St. Petersburg 199034, Russia.
Department of Mathematical and Information Technologies, Saint Petersburg Academic University, 8/3 Khlopina st., St. Petersburg 194021, Russia.
Proteomes. 2017 Feb 8;5(1):6. doi: 10.3390/proteomes5010006.
De novo sequencing of tandem (MS/MS) mass spectra represents the only way to determine the sequence of proteins from organisms with unknown genomes, or the ones not directly inscribed in a genome-such as antibodies, or novel splice variants. Top-down mass spectrometry provides new opportunities for analyzing such proteins; however, retrieving a complete protein sequence from top-down MS/MS spectra still remains a distant goal. In this paper, we review the state-of-the-art on this subject, and enhance our previously developed Twister algorithm for de novo sequencing of peptides from top-down MS/MS spectra to derive longer sequence fragments of a target protein.
串联（MS/MS）质谱的从头测序是确定来自基因组未知生物的蛋白质序列，或未直接记录在基因组中的蛋白质（如抗体或新型剪接变体）序列的唯一方法。自上而下的质谱分析为分析此类蛋白质提供了新的机会；然而，从自上而下的MS/MS谱图中检索完整的蛋白质序列仍然是一个遥远的目标。在本文中，我们综述了该主题的最新进展，并改进了我们之前开发的Twister算法，用于从自上而下的MS/MS谱图中对肽段进行从头测序，以获得目标蛋白质的更长序列片段。
