Botanical Institute, University of Cologne, Cologne Biocenter, 50674, Cologne, Germany.
Max-Planck Institute for Plant Breeding Research, 50829, Cologne, Germany.
Plant Cell Environ. 2017 Aug;40(8):1429-1441. doi: 10.1111/pce.12938. Epub 2017 Apr 27.
Leaf veins provide the mechanical support and are responsible for the transport of nutrients and water to the plant. High vein density is a prerequisite for plants to have C4 photosynthesis. We investigated the genetic variation and genetic architecture of leaf venation traits within the species Arabidopsis thaliana using natural variation. Leaf venation traits, including leaf vein density (LVD) were analysed in 66 worldwide accessions and 399 lines of the multi-parent advanced generation intercross population. It was shown that there is no correlation between LVD and photosynthesis parameters within A. thaliana. Association mapping was performed for LVD and identified 16 and 17 putative quantitative trait loci (QTLs) in the multi-parent advanced generation intercross and worldwide sets, respectively. There was no overlap between the identified QTLs suggesting that many genes can affect the traits. In addition, linkage mapping was performed using two biparental recombinant inbred line populations. Combining linkage and association mapping revealed seven candidate genes. For one of the candidate genes, RCI2c, we demonstrated its function in leaf venation patterning.
叶片的叶脉为植物提供机械支撑，并负责将营养物质和水分运输到植物体内。高的叶脉密度是植物具有 C4 光合作用的前提条件。我们利用自然变异，研究了拟南芥属内叶片脉序特征的遗传变异和遗传结构。对 66 个全球采集品系和 399 个多亲本高级世代互交群体的叶片脉序特征，包括叶片脉密度（LVD）进行了分析。结果表明，在拟南芥属内，LVD 与光合作用参数之间没有相关性。对 LVD 进行了关联作图，在多亲本高级世代互交和全球群体中分别鉴定出 16 个和 17 个假定的数量性状位点（QTL）。鉴定出的 QTL 之间没有重叠，这表明许多基因可以影响这些特征。此外，还利用两个双亲重组自交系群体进行了连锁作图。连锁和关联作图的结合揭示了七个候选基因。对于候选基因之一 RCI2c，我们证明了它在叶片脉序模式形成中的功能。
