Ling Shukuan, Zhong Guohui, Sun Weijia, Liang Fengji, Wu Feng, Li Hongxing, Li Yuheng, Zhao Dingsheng, Song Jinping, Jin Xiaoyan, Wu Xiaorui, Song Hailin, Li Qi, Li Yinghui, Chen Shanguang, Xiong Jianghui, Li Yingxian
State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center Beijing, China.
State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training CenterBeijing, China; Laboratory of Longitudinal Integration of Individual Life Data, Space Institute of Southern ChinaShenzhen, China.
Front Physiol. 2017 Feb 14;8:69. doi: 10.3389/fphys.2017.00069. eCollection 2017.
The purpose of this study was to find the circulating microRNAs (miRNAs) co-related with bone loss induced by bed rest, and testify whether the selected miRNAs could reflect the bone mineral status of human after bed-rest. We analyzed plasma miRNA levels of 16 subjects after 45 days of -6° head-down tilt bed rest, which is a reliable model for the simulation of microgravity. We characterize the circulating miRNA profile in individuals after bed rest and identify circulating miRNAs which can best reflect the level of bone loss induced by bed rest. Expression profiling of circulating miRNA revealed significant downregulation of 37 miRNAs and upregulation of 2 miRNAs, while only 11 of the downregulated miRNAs were further validated in a larger volunteer cohort using qPCR. We found that 10 of these 11 miRNAs (miR-103, 130a, 1234, 1290, 151-5p, 151-3p, 199a-3p, 20a, 363, and 451a) had ROC curve that distinguished the status after bed rest. Importantly, significant positive correlations were identified between bone loss parameters and several miRNAs, eventually miR-1234 showed clinical significance in detecting the bone loss of individuals after 45 days of bed rest.
本研究的目的是寻找与卧床休息引起的骨质流失相关的循环微RNA（miRNA），并验证所选的miRNA是否能反映卧床休息后人的骨矿物质状态。我们分析了16名受试者在-6°头低位卧床休息45天后的血浆miRNA水平，这是模拟微重力的可靠模型。我们对卧床休息后个体的循环miRNA谱进行了表征，并确定了最能反映卧床休息引起的骨质流失水平的循环miRNA。循环miRNA的表达谱显示37种miRNA显著下调，2种miRNA上调，而在更大的志愿者队列中使用qPCR进一步验证了仅11种下调的miRNA。我们发现这11种miRNA中的10种（miR-103、130a、1234、1290、151-5p、151-3p、199a-3p、20a、363和451a）具有区分卧床休息后状态的ROC曲线。重要的是，在骨质流失参数与几种miRNA之间发现了显著的正相关，最终miR-1234在检测45天卧床休息后个体的骨质流失方面显示出临床意义。
