Lennard A C, Egly J M
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France.
EMBO J. 1987 Oct;6(10):3027-34. doi: 10.1002/j.1460-2075.1987.tb02608.x.
The adenovirus-2 major late promoter (Ad2MLP) upstream element (Ad2MLP-UE) contains a sequence of interrupted dyad symmetry. By inverting this element we have found that it functions in a bidirectional manner both in vivo and in vitro. Footprinting and binding kinetics studies have demonstrated that both orientations of the upstream element bind the sequence-specific upstream factor (UEF) in a similar fashion. These data strongly suggest that the dyad symmetric sequence is sufficient for fully functional binding of the UEF. Binding studies of the UEF to the Ad2MLP-UE indicate that, contrary to prokaryotic palindromic promoter elements which bind multimers of specific factors, the entire Ad2MLP dyad symmetric upstream element binds a single monomeric UEF molecule.
腺病毒2型主要晚期启动子（Ad2MLP）上游元件（Ad2MLP-UE）包含一个间断的二元对称序列。通过将该元件倒置，我们发现在体内和体外它都以双向方式发挥作用。足迹法和结合动力学研究表明，上游元件的两个方向都以相似的方式结合序列特异性上游因子（UEF）。这些数据有力地表明二元对称序列足以实现UEF的全功能结合。UEF与Ad2MLP-UE的结合研究表明，与结合特定因子多聚体的原核回文启动子元件相反，整个Ad2MLP二元对称上游元件结合单个单体UEF分子。
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