新型隐球菌中富含磷的结构与荚膜结构
Phosphorus-rich structures and capsular architecture in Cryptococcus neoformans.
作者信息
Ramos Caroline L, Gomes Fabio M, Girard-Dias Wendell, Almeida Fernando P, Albuquerque Priscila C, Kretschmer Matthias, Kronstad James W, Frases Susana, de Souza Wanderley, Rodrigues Marcio L, Miranda Kildare
机构信息
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
出版信息
Future Microbiol. 2017 Mar;12:227-238. doi: 10.2217/fmb-2017-0060. Epub 2017 Feb 20.
AIM
In this study, we aimed to analyze the relationship of phosphorus-rich structures with surface architecture in Cryptococcus neoformans.
METHODS
Phosphorus-rich structures in C. neoformans were analyzed by combining fluorescence microscopy, biochemical extraction, scanning electron microscopy, electron probe x-ray microanalysis and 3D reconstruction of high pressure frozen and freeze substituted cells by focused ion beam-scanning electron microscopy (FIB-SEM).
RESULTS & CONCLUSION: Intracellular and surface phosphorus-enriched structures were identified. These molecules were required for capsule assembly, as demonstrated in experiments using polysaccharide incorporation by capsule-deficient cells and mutants with defects in polyphosphate synthesis. The demonstration of intracellular and cell wall-associated polyphosphates in C. neoformans may lead to future studies involving their participation in both physiologic and pathogenic events.
目的
在本研究中，我们旨在分析新型隐球菌中富含磷的结构与表面结构的关系。
方法
通过结合荧光显微镜、生化提取、扫描电子显微镜、电子探针X射线微分析以及聚焦离子束扫描电子显微镜（FIB-SEM）对高压冷冻和冷冻置换细胞进行三维重建，来分析新型隐球菌中富含磷的结构。
结果与结论
鉴定出细胞内和表面富含磷的结构。如在使用缺乏荚膜的细胞掺入多糖以及多聚磷酸盐合成存在缺陷的突变体的实验中所证明的，这些分子是荚膜组装所必需的。新型隐球菌中细胞内和细胞壁相关多聚磷酸盐的证明可能会引发未来关于它们参与生理和致病事件的研究。
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