Gardette J, Margelin D, Maziere J C, Bertrand J, Picard J
INSERM U181, Faculté de Médecine Saint-Antoine, Paris, France.
FEBS Lett. 1987 Dec 10;225(1-2):178-82. doi: 10.1016/0014-5793(87)81153-5.
The effect of dibutyryl cyclic AMP and theophylline on lipoprotein lipase secretion was investigated after a 24 h pretreatment of human monocyte-derived macrophages. Both the effectors decreased in a dose-dependent manner the enzyme activity recovered in the culture medium. The decrease in lipoprotein lipase activity appeared to be related to reduced enzyme synthesis without apparent modification of its stability and half-life and was conversely associated with an increase of lysosomal acid hydrolase activities. This effect was reversible on removal of the nucleotide. The present findings suggest that cyclic AMP may play a role in lipoprotein lipase expression in human macrophages and therefore may participate in the regulation of lipoprotein uptake by these cells, which are strongly implicated in the atherogenic process.
在对人单核细胞衍生的巨噬细胞进行24小时预处理后，研究了二丁酰环磷腺苷和茶碱对脂蛋白脂肪酶分泌的影响。两种效应物均以剂量依赖性方式降低了培养基中恢复的酶活性。脂蛋白脂肪酶活性的降低似乎与酶合成减少有关，而其稳定性和半衰期没有明显改变，相反，与溶酶体酸性水解酶活性的增加有关。去除核苷酸后，这种效应是可逆的。目前的研究结果表明，环磷腺苷可能在人巨噬细胞中脂蛋白脂肪酶的表达中起作用，因此可能参与这些细胞对脂蛋白摄取的调节，而这些细胞在动脉粥样硬化过程中起着重要作用。
