Schreiner Bernadette, Ankarcrona Maria
Center for Alzheimer Research, Division of Neurogeriatrics, Department of Neurobiology, Care Sciences and Society,, Karolinska Institutet, SE, -141 57, Huddinge, Sweden.
Methods Mol Biol. 2017;1567:53-68. doi: 10.1007/978-1-4939-6824-4_5.
During the last decades, increasing evidence indicated that subcellular organelles do not exist as autarkic units but instead communicate constantly and extensively with each other in various ways. Some communication, for example, the exchange of small molecules, requires the marked convergence of two distinct organelles for a certain period of time. The cross talk between endoplasmic reticulum (ER) and mitochondria, two subcellular organelles of utmost importance for cellular bioenergetics and protein homeostasis, has been increasingly investigated under the last years. This development was significantly driven by the establishment of optimized subcellular fractionation techniques. In this chapter, we will describe and critically discuss the currently used protocol for the isolation of the membrane fraction containing mitochondria-associated membranes (MAM).
在过去几十年中，越来越多的证据表明，亚细胞器并非作为自给自足的单位存在，而是以各种方式持续且广泛地相互交流。例如，一些交流，如小分子的交换，需要两个不同的细胞器在一段时间内显著靠近。内质网（ER）和线粒体这两个对细胞生物能量学和蛋白质稳态至关重要的亚细胞器之间的相互作用，在过去几年中受到了越来越多的研究。这一进展很大程度上得益于优化的亚细胞分级分离技术的建立。在本章中，我们将描述并批判性地讨论目前用于分离包含线粒体相关膜（MAM）的膜组分的方案。
