重组VP1蛋白作为诊断甲型肝炎病毒急性感染的潜在标志物。
Recombinant VP1 protein as a potential marker for the diagnosis of acute hepatitis A virus infection.
作者信息
da Silva Junior Haroldo Cid, da Silva Edimilson Domingos, Lewis-Ximenez de Souza Rodrigues Lia Laura, Medeiros Marco Alberto
机构信息
Laboratório de Tecnologia Recombinante, Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos), Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, RJ, Brazil.
Laboratório de Tecnologia Diagnóstica, Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos), Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, RJ, Brazil.
出版信息
J Virol Methods. 2017 Jul;245:1-4. doi: 10.1016/j.jviromet.2017.03.005. Epub 2017 Mar 9.
Since hepatitis A virus (HAV) production is time-consuming and expensive, the use of recombinant proteins may represent an alternative source of antigens for diagnostic purposes. The present study aimed to express, purify and evaluate the potential of recombinant VP1 protein (rVP1) as a marker for the diagnosis of acute HAV infection. The rVP1 was expressed and purified successfully from Escherichia coli. The purified rVP1 was used to establish an in-house enzyme-linked immunosorbent assay (ELISA-rVP1) for detection of IgM antibodies in sera from HAV-positive patients. For a cut-off point of 0.351, the sensitivity and specificity of ELISA-rVP1 were 100.0% and 95.0%, respectively. These results indicate that rVP1 may be a useful antigen for detection of IgM antibodies against HAV.
由于甲型肝炎病毒（HAV）的生产耗时且昂贵，使用重组蛋白可能是用于诊断目的的抗原的替代来源。本研究旨在表达、纯化并评估重组VP1蛋白（rVP1）作为诊断急性HAV感染标志物的潜力。rVP1成功从大肠杆菌中表达并纯化。纯化后的rVP1用于建立一种内部酶联免疫吸附测定法（ELISA-rVP1），以检测HAV阳性患者血清中的IgM抗体。对于0.351的截断点，ELISA-rVP1的敏感性和特异性分别为100.0%和95.0%。这些结果表明，rVP1可能是检测抗HAV IgM抗体的有用抗原。
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