Fejzić Elma, Karamehić Jasenko, Eminović Izet, Suljević Damir, Alijagić Andi, Bećirević Semir, Šahović Amela, Šišić Sanela
Institute of Transfusion Medicine of Federation of Bosnia and Herzegovina, Sarajevo, Bosnia and Herzegovina.
Department of Immunology, Clinical Center University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
Open Access Maced J Med Sci. 2017 Mar 15;5(1):1-5. doi: 10.3889/oamjms.2017.015. Epub 2017 Jan 30.
The research was conducted by genotyping two Human Leukocyte Antigen (HLA) gene classes. The main objective of this research was to investigate distribution and frequency of the allelic groups, genotypes and haplotypes in the gene loci of HLA class I (HLA-A*, -B*, -C*) and HLA class II (HLA-DRB1*, -DQB1*) in patients included in the program of cadaveric renal transplantation.
Our study covered 186 blood samples of patients who are registered on the list for cadaveric renal transplantation in Federation of Bosnia and Herzegovina and included 59 control, healthy unrelated individuals. For the HLA typing, we have used three different methods: micro lymphocyte cytotoxicity test (MLCT), Polymerase Chain Reaction (PCR) - Sequence Specific Primers (SSP) and PCR - Sequence-Specific Oligonucleotides (SSO) or Luminex technology. All patients and cadaveric donors were tested using the three methods because the system is polymorphic.
Analysis of the results of genotyping HLA class I gene loci identified dominant HLA-A02, HLA-B35, HLA-C07 allelic groups. Analysis of the HLA class II gene loci genotyping showed that HLA-DRB111 and HLA-DQB1*03 loci had the highest incidence in HLA class II.
Based on our results and previous research, there were no observed differences between allelic frequencies and genotypes of healthy people and people with ESRD. Differences between allelic groups occurred, but they were not statistically significant, except HLA-C*01 (p = 0.020).
本研究通过对两类人类白细胞抗原（HLA）基因进行基因分型展开。本研究的主要目的是调查尸体肾移植项目中患者的HLA I类基因座（HLA-A*、-B*、-C*）和HLA II类基因座（HLA-DRB1*、-DQB1*）的等位基因组、基因型和单倍型的分布及频率。
我们的研究涵盖了波斯尼亚和黑塞哥维那联邦尸体肾移植登记名单上的186例患者的血样，并纳入了59名对照健康无关个体。对于HLA分型，我们使用了三种不同的方法：微量淋巴细胞毒性试验（MLCT）、聚合酶链反应（PCR）-序列特异性引物（SSP）以及PCR-序列特异性寡核苷酸（SSO）或Luminex技术。所有患者和尸体供者均使用这三种方法进行检测，因为该系统具有多态性。
对HLA I类基因座基因分型结果的分析确定了优势等位基因组HLA-A02、HLA-B35、HLA-C07。对HLA II类基因座基因分型的分析表明，HLA-DRB111和HLA-DQB1*03基因座在HLA II类中发病率最高。
基于我们的研究结果和先前的研究，未观察到健康人与终末期肾病患者之间在等位基因频率和基因型上存在差异。等位基因组之间存在差异，但除HLA-C*01外（p = 0.020），差异无统计学意义。
