与猫外周血单个核细胞交叉反应的鼠单克隆抗体。

Murine monoclonal antibodies cross-reactive with feline peripheral blood mononuclear cells.

作者信息

Shelton G H, Ladiges W C

机构信息

Pacific Northwest Research Foundation, Seattle, WA.

出版信息

Vet Immunol Immunopathol. 1987 Nov;16(3-4):271-6. doi: 10.1016/0165-2427(87)90023-7.

DOI:10.1016/0165-2427(87)90023-7

PMID:2829413

Abstract

Twelve murine monoclonal antibodies (MAB), specific for canine, human, and mouse cell surface determinants on lymphohemopoietic cells, were tested for reactivity (using indirect immunofluorescence) with peripheral blood mononuclear cells (PBMC) from 3 normal cats and 3 FeLV-positive cats with lymphoblastic leukemia. MAB DLy6 and 1H3, specific for canine lymphocytes, MAB HB57S, specific for human mu(IgM) chain, MAB J-118 40.164.3 and H81.98.71, specific for murine and human Ia, all had higher binding levels with PBMC from FeLV-positive cats compared to normal cats. Two MAB (S-78, S-24), specific for human class I determinants, cross-reacted with PBMC from both groups of cats. This panel of MAB may be useful for characterizing immunologically reactive cell subsets in normal as well as retrovirus-diseased animals.

摘要

针对淋巴造血细胞上犬、人及小鼠细胞表面决定簇的12种鼠单克隆抗体（MAB），采用间接免疫荧光法检测其与3只正常猫和3只患淋巴细胞白血病的FeLV阳性猫外周血单个核细胞（PBMC）的反应性。针对犬淋巴细胞的MAB DLy6和1H3、针对人μ（IgM）链的MAB HB57S、针对鼠和人Ia的MAB J-118 40.164.3和H81.98.71，与FeLV阳性猫的PBMC的结合水平均高于正常猫。两种针对人I类决定簇的MAB（S-78、S-24）与两组猫的PBMC均发生交叉反应。这组MAB可能有助于鉴定正常及逆转录病毒感染患病动物中具有免疫反应性的细胞亚群。