利用小角X射线散射（SAXS）表征基质金属蛋白酶（MMPs）的溶液构象和柔韧性。

Using Small Angle X-Ray Scattering (SAXS) to Characterize the Solution Conformation and Flexibility of Matrix Metalloproteinases (MMPs).

作者信息

Butt Louise E, Holland Robert A, Khunti Nikul S, Quinn Debra L, Pickford Andrew R

机构信息

Institute of Biomedical and Biomolecular Science (IBBS) and School of Biological Sciences, University of Portsmouth, King Henry Building, King Henry I Street, Portsmouth, PO1 2DY, UK.

Diamond Light Source, Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, UK.

出版信息

Methods Mol Biol. 2017;1579:87-108. doi: 10.1007/978-1-4939-6863-3_6.

DOI:10.1007/978-1-4939-6863-3_6

PMID:28299734

Abstract

Small angle X-ray scattering (SAXS) provides information about the conformation and flexibility of proteins in solution, and hence provides complementary structural information to that obtained from X-ray crystallography and nuclear magnetic resonance spectroscopy. In this chapter, we describe the methods for the preparation of matrix metalloproteinase (MMP) samples for SAXS analyses, and for the acquisition, processing and interpretation of the SAXS data.

摘要

小角X射线散射（SAXS）可提供有关溶液中蛋白质构象和柔韧性的信息，因此能为从X射线晶体学和核磁共振光谱学获得的结构信息提供补充。在本章中，我们描述了用于SAXS分析的基质金属蛋白酶（MMP）样品的制备方法，以及SAXS数据的采集、处理和解释方法。