School of Nursing, Chengdu Medical College, Chengdu, Sichuan, 610083, China.
School of Nursing, Third Military Medical University, Chongqing, 400038, China.
Probiotics Antimicrob Proteins. 2017 Jun;9(2):172-181. doi: 10.1007/s12602-017-9266-x.
The main objective of this study was to investigate the effects of Synbiotic2000™ Forte on the intestinal motility and interstitial cells of Cajal (ICC) in traumatic brain injury (TBI) mouse model. Kunming mice were randomly divided into sham operation group (S group), enteral nutrition group with TBI (E group), and Synbiotic2000™ Forte group with TBI (P group). The contractile activity of the intestinal smooth muscle, densities and ultrastructure of the ICC, kit protein concentration, weight, and defecation of mice were monitored and analyzed. TBI markedly suppressed contractile activity of the intestinal smooth muscle (P < 0.01), which led to a reduction of defecation (P < 0.01) and weight (P < 0.01). However, application of Synbiotic2000™ Forte significantly improved contractile activity of the small intestine (P < 0.01), which may be related to protective effects to the interstitial cells of Cajal, smooth muscle cells, and enteric neurons. TBI impaired ICC networks and densities (P < 0.01), events that were protected by the application of Synbiotic2000™ Forte. Synbiotic2000™ Forte may attenuate TBI-mediated inhibition of the kit protein pathway. Synbiotic2000™ Forte may improve intestinal motility and protect the ICC in the TBI mouse. These findings provide a novel support for the application of Synbiotic2000™ Forte in intestinal motility disturbance after TBI.
本研究的主要目的是探讨合生元 2000 Forte 对创伤性脑损伤（TBI）小鼠模型肠道动力和 Cajal 间质细胞（ICC）的影响。昆明小鼠随机分为假手术组（S 组）、TBI 肠内营养组（E 组）和 TBI 合生元 2000 Forte 组（P 组）。监测和分析了肠道平滑肌的收缩活性、ICC 的密度和超微结构、kit 蛋白浓度、小鼠体重和排便情况。TBI 明显抑制了肠道平滑肌的收缩活性（P<0.01），导致排便减少（P<0.01）和体重减轻（P<0.01）。然而，合生元 2000 Forte 的应用显著改善了小肠的收缩活性（P<0.01），这可能与对 Cajal 间质细胞、平滑肌细胞和肠神经元的保护作用有关。TBI 损害了 ICC 网络和密度（P<0.01），而合生元 2000 Forte 的应用则保护了这些 ICC。合生元 2000 Forte 可能减轻了 TBI 介导的 kit 蛋白通路的抑制。合生元 2000 Forte 可改善 TBI 小鼠的肠道动力并保护 ICC。这些发现为合生元 2000 Forte 在 TBI 后肠道动力障碍中的应用提供了新的支持。
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