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[海葵触手性息肉的测定。生物学和化学分析]

[Determination of sexual polyps in hydractinia echinata. A biological and chemical analysis].

作者信息

Müller Werner Alois

机构信息

Laboratory of the Marine Biological Association of the United Kingdom, Plymouth.

Zoologisches Institut der Technischen Universität Braunschweig, Deutschland.

出版信息

Wilhelm Roux Arch Entwickl Mech Org. 1969 Mar;164(1):37-47. doi: 10.1007/BF00577680.

Abstract
  1. In contact with tissue of blastostyles buds of gasterozooids and regenerating gasterozooids will transform into blastostyles. The inductive capacity within blastostyles decreases from apex to base. Hypostomes with gasterozooid-like properties, which may frequently occur at the basal cut of isolated blastostyles, will transform into the homologous blastostyle structures in a sequence following that inductive gradient. 2. The spontaneous transformation of regenerating gasterozooids into reproductive polyps, which could be observed during wintertime, can be increased by α-ketoglutarate (5×10 M/L), isocitrate (5×10 M/L), colcemide (3×10 M/L) and, with only weak effect, by CO increased top=2-4% Atm. With increasing dosages optimum lines could be determined. The transformable state of regeneration starts when tentacle formation takes place. By applying α-ketoglutarate in stepwise decreased concentrations 75 ppc transformations have been obtainable. 3. If similarly applied in graduated dosages chromatographically separated blastostyle ectraxts also increase the rate of transformation. The causative factor is to be found in the range of fractions that also contains the tentacle-inducing substance.
摘要
  1. 与胃腔类芽体的组织接触时,再生的胃腔类会转化为芽茎。芽茎内的诱导能力从顶端到底部逐渐降低。在分离的芽茎基部切口处经常出现的具有类似胃腔类特性的口管,会按照该诱导梯度依次转化为同源的芽茎结构。2. 在冬季可观察到再生的胃腔类自发转化为生殖息肉,α-酮戊二酸(5×10⁻³摩尔/升)、异柠檬酸(5×10⁻³摩尔/升)、秋水仙酰胺(3×10⁻³摩尔/升)以及二氧化碳浓度增加到2-4%大气压可增强这种转化,不过二氧化碳的效果较弱。随着剂量增加可确定最佳剂量线。再生的可转化状态在触手形成时开始。通过逐步降低α-酮戊二酸的浓度,已实现75%的转化率。3. 以分级剂量类似地应用色谱分离的芽茎提取物,也会提高转化速率。致病因素存在于也含有触手诱导物质的组分范围内。

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