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果蝇卵壳在化学成分明确的器官培养基中的体外发育。

In vitro development of theDrosophila chorion in a chemically defined organ culture medium.

作者信息

Petri William H, Mindrinos Mihalis N, Lombard Mary F, Margaritis Lukas H

机构信息

Biology Department, Boston College, 02167, Chestnut Hill, Massachusetts, USA.

Department of Biology, University of Athens, Panepistemiopolis, Kouponia, (621), Athens, Greece.

出版信息

Wilehm Roux Arch Dev Biol. 1979 Dec;186(4):351-362. doi: 10.1007/BF00848458.

Abstract

TheDrosophila chorion is produced normally in isolated follicles in Robb's chemically defined culture medium. The complex architecture of the shell developed in vitro from follicles as young as early stage 10 is completely normal morphologically. In addition, the time required for in vitro development closely approximates that observed for in vivo development. Comparisons of insect culture media developed by Robb, Grace, Schneider, and Echalier show large variations in their ability to supportDrosophila chorion development.

摘要

果蝇卵壳在罗布化学限定培养基中的分离卵泡中正常产生。在体外从早期10阶段的卵泡发育而来的壳的复杂结构在形态上完全正常。此外,体外发育所需的时间与体内发育观察到的时间非常接近。对罗布、格雷斯、施奈德和埃沙利尔开发的昆虫培养基的比较表明,它们支持果蝇卵壳发育的能力有很大差异。

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