对黑腹果蝇雌性性别发育所需基因性致死基因的克隆分析。
Clonal analysis ofsex-lethal, a gene needed for female sexual development inDrosophila melanogaster.
作者信息
Sánchez Lucas, Nöthiger Rolf
机构信息
Zoologisches Institut der Universität Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland.
出版信息
Wilehm Roux Arch Dev Biol. 1982 May;191(3):211-214. doi: 10.1007/BF00848339.
The mutationSxl , located on the X-chromosome, is a sex-limited recessive lethal that specifically kills 2X; 2A flies while it does not affect X; 2A flies (Cline 1978). We have analyzed the role ofSxl on sex determination by a clonal analysis of a new spontaneous allele,Sxl . Female embryos and larvae heterozygous forSxl were irradiated at different times of development to generate homozygousSxl clones which were recognized by linked marker mutations. We have studied the phenotype of such clones on sexually dimorphic regions of the fly (foreleg basitarsus, 5th, 6th and 7th tergites, analia and external genitalia). Despite their female (2X; 2A) chromosomal constitution, clones homozygous forSxl differentiated male structures. These results confirm and extend the preliminary report of Cline (1979). They show that the wildtype product ofSxl is required for female development.
位于X染色体上的突变基因Sxl是一种性别限制的隐性致死基因，它能特异性地杀死2X; 2A的果蝇，而不影响X; 2A的果蝇（克莱因，1978年）。我们通过对一个新的自发等位基因Sxl进行克隆分析，研究了Sxl在性别决定中的作用。对发育不同阶段的Sxl杂合雌性胚胎和幼虫进行辐照，以产生通过连锁标记突变识别的纯合Sxl克隆。我们研究了这些克隆在果蝇性二态区域（前腿跗节基部、第5、6和7腹节、肛区和外生殖器）的表型。尽管其染色体组成为雌性（2X; 2A），但Sxl纯合克隆却分化出雄性结构。这些结果证实并扩展了克莱因（1979年）的初步报告。它们表明，Sxl的野生型产物是雌性发育所必需的。
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