Kühtreiber Wiel M, van der Bent Jos, Dorresteijn Adrie W C, de Graaf Arjan, van den Biggelaar Jo A M, van Dongen Cees A M
Department of Experimental Zoology, University of Utrecht, Padualaan 8, 3508 TB, Utrecht, The Netherlands.
Rouxs Arch Dev Biol. 1986 May;195(4):265-275. doi: 10.1007/BF02438960.
In 32-cell stage embryos ofPatella vulgata one of the macromeres contacts the animal micromeres, and as a result is induced to differentiate into the stem cell of the mesodermal cell line. In this study we show the presence of an extracellular matrix (ECM) between these two interacting cell types. The ECM appears to be formed by the micromeres during the 32-cell stage. Staining experiments with alcian blue and tannic acid indicate that in contains glycoconjugates, possibly in the form of proteoglycans. The characteristics of the ECM were examined further by fluorescein isothiocyanate (FITC)-lectin labelling. Of 17 lectins tested, concanavalin A (ConA), succinyl-ConA, LCH-B (Lens culinaris) and PEA (Pisum sativum) showed a positive labelling of the ECM. These results are in accordance with the electron microscopic data. The appearance of the ECM at this specific stage and place suggests that it might play an important role in the induction of the mesodermal cell line.
在紫贻贝32细胞期胚胎中，其中一个大卵裂球与动物极微卵裂球接触，结果被诱导分化为中胚层细胞系的干细胞。在本研究中，我们展示了这两种相互作用的细胞类型之间存在细胞外基质（ECM）。ECM似乎是在32细胞期由微卵裂球形成的。用阿尔新蓝和单宁酸进行的染色实验表明，它含有糖缀合物，可能是以蛋白聚糖的形式存在。通过异硫氰酸荧光素（FITC）凝集素标记进一步研究了ECM的特性。在测试的17种凝集素中，伴刀豆球蛋白A（ConA）、琥珀酰-ConA、扁豆凝集素（LCH-B）和豌豆凝集素（PEA）对ECM呈阳性标记。这些结果与电子显微镜数据一致。ECM在这个特定阶段和位置的出现表明它可能在中胚层细胞系的诱导中起重要作用。
