伤寒沙门氏菌孔蛋白基因在大肠杆菌中的分子克隆与表达
Molecular cloning and expression in E. coli of a Salmonella typhi porin gene.
作者信息
Zaror I, Gómez I, Castillo G, Yudelevich A, Venegas A
机构信息
Laboratorio de Bioquímica, Pontificia Universidad Católica de Chile, Santiago.
出版信息
FEBS Lett. 1988 Feb 29;229(1):77-81. doi: 10.1016/0014-5793(88)80801-9.
PMID:2831092
Abstract
Immunoscreening of a Salmonella typhi cosmid library in E. coli allowed the detection of clones producing a 36 kDa porin from S. typhi. The gene is efficiently expressed in an E. coli porin-less mutant and the protein is exported to the outer membrane envelope. Two clones which markedly differ in their level of expression have been isolated.
摘要
对大肠杆菌中伤寒沙门氏菌黏粒文库进行免疫筛选，能够检测到产生来自伤寒沙门氏菌的36 kDa孔蛋白的克隆。该基因在无孔蛋白的大肠杆菌突变体中高效表达，且该蛋白被转运至外膜包膜。已分离出两个表达水平明显不同的克隆。
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