Hensen J, Hader O, Bähr V, Oelkers W
Department of Internal Medicine, Klinikum Steglitz, Freie Universität Berlin, West Germany.
J Clin Endocrinol Metab. 1988 Apr;66(4):668-71. doi: 10.1210/jcem-66-4-668.
Arginine vasopressin (AVP) regulates ACTH release under certain conditions, and exogenously administered AVP is used clinically to stimulate ACTH secretion. We attempted to determine at what plasma concentration AVP can stimulate ACTH release. Six normal men were given infusions of AVP (Ferring) or vehicle between 1600 and 1700 h on five occasions: 1) saline (30 mL/h); 2) 10 ng AVP/min; 3) 30 ng AVP/min; 4) 100 ng AVP/min; and 5) 300 ng AVP/min. Plasma AVP, ACTH, and cortisol concentrations were measured every 10 min during the infusions. Basal plasma AVP levels were less than 1 ng/L (less than 0.92 pmol/L). The lowest AVP dose raised plasma AVP into the range found in fluid-deprived subjects (7-8 ng/L;6.5-7.3 pmol/L), but had no effect on plasma ACTH concentrations. AVP in a dose of 30 ng/min also had no effect. The 100 ng AVP/min dose raised plasma AVP concentrations to 51.4-65.5 ng/L (46-60 pmol/L). This increase led to a transient insignificant increase in plasma ACTH from 13.9 +/- 1.2 (+/- SEM) ng/L (3.1 +/- 0.3 pmol/L) to 20.0 +/- 1.4 ng/L (4.4 +/- 0.3 pmol/L), while plasma cortisol rose significantly from 146 +/- 10 to 209 +/- 19 nmol/L (P less than 0.01) after 60 min of infusion. The 300 ng AVP/min dose raised plasma AVP levels to about 260 ng/L (239 pmol/L); the maximal plasma ACTH and cortisol levels were 39.5 +/- 5.0 ng/L (8.7 +/- 1.1 pmol/L; P less than 0.01) and 348 nmol/L (P less than 0.01), respectively. Thus, peripheral plasma AVP levels have to be raised high above the physiological range before ACTH release is stimulated. We conclude that any AVP reaching the adenohypophysis through the peripheral circulation is of much less importance for the regulation of ACTH secretion than is AVP derived from the pituitary portal circulation.
精氨酸加压素（AVP）在某些情况下调节促肾上腺皮质激素（ACTH）的释放，外源性给予的AVP在临床上用于刺激ACTH分泌。我们试图确定AVP在何种血浆浓度下能够刺激ACTH释放。六名正常男性在五个不同时间段（1600至1700时）接受了AVP（Ferring）或赋形剂输注：1）生理盐水（30 mL/h）；2）10 ng AVP/分钟；3）30 ng AVP/分钟；4）100 ng AVP/分钟；5）300 ng AVP/分钟。在输注过程中每隔10分钟测量血浆AVP、ACTH和皮质醇浓度。基础血浆AVP水平低于1 ng/L（低于0.92 pmol/L）。最低剂量AVP使血浆AVP升高至缺水受试者的水平范围（7 - 8 ng/L；6.5 - 7.3 pmol/L），但对血浆ACTH浓度无影响。30 ng/分钟剂量的AVP也无作用。100 ng AVP/分钟剂量使血浆AVP浓度升高至51.4 - 65.5 ng/L（46 - 60 pmol/L）。这种升高导致血浆ACTH从13.9 ± 1.2（± SEM）ng/L（3.1 ± 0.3 pmol/L）短暂且不显著地升高至20.0 ± 1.4 ng/L（4.4 ± 0.3 pmol/L），而在输注60分钟后血浆皮质醇从146 ± 10显著升高至209 ± 19 nmol/L（P < 0.01）。300 ng AVP/分钟剂量使血浆AVP水平升高至约260 ng/L（239 pmol/L）；血浆ACTH和皮质醇的最高水平分别为39.5 ± 5.0 ng/L（8.7 ± 1.1 pmol/L；P < 0.01）和348 nmol/L（P < 0.01）。因此，在外周血浆AVP水平升高至远高于生理范围之前，ACTH释放不会受到刺激才会被刺激。我们得出结论，通过外周循环到达腺垂体的任何AVP对于ACTH分泌调节的重要性远低于来自垂体门脉循环的AVP。
