Hingole A C, Gudewar J G, Pednekar R P, Gatne M L
Department of Veterinary Parasitology, Bombay Veterinary College, Parel, Mumbai, 400012 India.
J Parasit Dis. 2017 Mar;41(1):131-136. doi: 10.1007/s12639-016-0763-8. Epub 2016 Apr 7.
Faecal samples of cattle and buffaloes of Mumbai region collected between November 2012 to June 2013 were analysed by conventional and molecular tools to note the prevalence of cryptosporidiosis and species involved in the infection. Conventional analysis viz., direct faecal smear examination, faecal smear examination after normal saline sedimentation, Sheather's floatation and Sheather's floatation sedimentation smear methods demonstrated oocysts of in 141 (36.06 %) of 391 samples with higher occurrence in buffaloes (36.99 %) than cattle (34.48 %). Diarrhoeic loose faeces showed higher prevalence (42.07 %) than apparently normal faeces (31.72 %) irrespective of the host species. When data were arranged as per age groups viz., calves of 0-1 month, 1-2 months, 2-3 months and adults, the highest prevalence was noted in the youngest group (47.12 %) declining gradually with the advancing age with lowest (6.25 %) in adults indicating inverse correlation between prevalence rate and age of the host. These differences were statistically significant in case of buffaloes. was tentatively identified by morphometric analysis. By employing molecular tools like nested PCR, PCR-RFLP and sequence analysis of few samples showed good correlation in the identification of species of involved in the infection and demonstrated occurrence of , and Thus all the four commonly occurring bovine species of were encountered in the study area which appears to be a first record reporting the occurrence of with species level identification in large ruminants from Western region of India. Additionally, the public health significance of was also discussed in light of epidemiological factors pertaining to the region.
对2012年11月至2013年6月期间收集的孟买地区牛和水牛的粪便样本，采用传统方法和分子工具进行分析，以了解隐孢子虫病的流行情况及感染所涉及的种类。传统分析方法，即直接粪便涂片检查、生理盐水沉淀后粪便涂片检查、谢弗氏漂浮法和谢弗氏漂浮沉淀涂片法，在391份样本中的141份（36.06%）中检测到卵囊，水牛（36.99%）中的发生率高于牛（34.48%）。无论宿主种类如何，腹泻稀便的患病率（42.07%）均高于外观正常的粪便（31.72%）。当按年龄组（即0 - 1个月、1 - 2个月、2 - 3个月的犊牛和成年牛）整理数据时，最年幼组的患病率最高（47.12%），随着年龄增长逐渐下降，成年牛中最低（6.25%），表明患病率与宿主年龄呈负相关。这些差异在水牛中具有统计学意义。通过形态学分析初步鉴定了隐孢子虫。通过采用巢式PCR、PCR - RFLP等分子工具以及对少数样本的序列分析，在感染所涉及的隐孢子虫种类鉴定中显示出良好的相关性，并证明存在微小隐孢子虫、安氏隐孢子虫和牛隐孢子虫。因此，在研究区域中发现了所有四种常见的牛隐孢子虫种类，这似乎是首次在印度西部地区大型反刍动物中报告隐孢子虫种类水平鉴定的记录。此外，还根据该地区的流行病学因素讨论了隐孢子虫的公共卫生意义。
