豚鼠胃分散主细胞上胆囊收缩素和胃泌素功能不同的受体

Functionally distinct receptors for cholecystokinin and gastrin on dispersed chief cells from guinea pig stomach.

作者信息

Cherner J A, Sutliff V E, Grybowski D M, Jensen R T, Gardner J D

机构信息

Digestive Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.

出版信息

Am J Physiol. 1988 Feb;254(2 Pt 1):G151-5. doi: 10.1152/ajpgi.1988.254.2.G151.

DOI:10.1152/ajpgi.1988.254.2.G151

PMID:2831729

Abstract

Caerulein, gastrin, and C-terminal fragments of cholecystokinin (CCK) varying in length from eight (CCK-8) to four (CCK-4) amino acids stimulate pepsinogen secretion from dispersed chief cells prepared from guinea pig stomach. C-terminal fragments of CCK containing fewer than four amino acids, even when tested at concentrations as high as 3 mM, do not stimulate pepsinogen secretion. The efficacies of gastrin and the various CCK-related peptides, coupled with the pattern of action of CCK receptor antagonists, indicate that chief cells from guinea pig stomach possess two functionally distinct classes of receptors, C-receptors and G-receptors. The C-receptors can be occupied by caerulein, CCK-8, CCK-7, des(SO3)CCK-8, or des(SO3)CCK-7, and occupation of C-receptors causes full stimulation of pepsinogen secretion. G-receptors can be occupied by gastrin I, gastrin II, CCK-6, CCK-5, or CCK-4, and occupation of G-receptors causes stimulation of pepsinogen secretion that is 60% of maximal.

摘要

蛙皮素、胃泌素以及胆囊收缩素（CCK）的C末端片段（长度从8个氨基酸（CCK-8）到4个氨基酸（CCK-4）不等）可刺激从豚鼠胃制备的分散主细胞分泌胃蛋白酶原。CCK的C末端片段若含少于4个氨基酸，即便在高达3 mM的浓度下进行测试，也不会刺激胃蛋白酶原分泌。胃泌素及各种CCK相关肽的效力，再加上CCK受体拮抗剂的作用模式，表明豚鼠胃的主细胞拥有两类功能不同的受体，即C受体和G受体。C受体可被蛙皮素、CCK-8、CCK-7、去（SO3）CCK-8或去（SO3）CCK-7占据，C受体被占据会导致胃蛋白酶原分泌完全受到刺激。G受体可被胃泌素I、胃泌素II、CCK-6、CCK-5或CCK-4占据，G受体被占据会导致胃蛋白酶原分泌受到刺激，其刺激程度为最大值的60%。