Shan Xueling, Yamauchi Takuya, Yamamoto Yojiro, Niyomdecha Saroh, Ishiki Kengo, Le Dung Q, Shiigi Hiroshi, Nagaoka Tsutomu
Department of Applied Chemistry, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan.
Chem Commun (Camb). 2017 Mar 30;53(27):3890-3893. doi: 10.1039/c7cc00244k.
Specific identification of enterohemorrhagic Escherichia coli was achieved using microspheres coated with overoxidized polypyrrole. The microspheres are well dispersed in aqueous media, and they specifically, spontaneously, and efficiently bind E. coli O157:H7 through surface area effects. In addition, we found that light-scattering by a single microsphere depended linearly on the number of bound cells.
使用涂覆有过氧化聚吡咯的微球实现了肠出血性大肠杆菌的特异性鉴定。这些微球在水性介质中分散良好,并且通过表面积效应特异性地、自发地和高效地结合大肠杆菌O157:H7。此外,我们发现单个微球的光散射与结合细胞的数量呈线性关系。