一种使用快速蛋白质色谱法以酶原形式纯化补体亚成分C1r和C1s的快速高效方法。
A rapid and efficient method for the purification of the complement subcomponents C1r and C1s in zymogen form using fast protein chromatography.
作者信息
Peitsch M C, Kovacsovics T J, Isliker H
机构信息
Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
出版信息
J Immunol Methods. 1988 Apr 6;108(1-2):265-9. doi: 10.1016/0022-1759(88)90428-0.
The purification of the subcomponents C1r and C1s of the first component of complement involves multiple steps and is time-consuming. This accounts for the frequently observed partial activation of the subcomponents. In this report we propose a simplified procedure of purification using a batch method and fast protein chromatography avoiding a shift of pH. The method provides C1r and C1s in a yield of 35 and 60% respectively. In addition, this study provides a simple and sensitive test to assess functional purity of C1r and C1s with respect to the other C1 subcomponents.
补体第一成分的亚组分C1r和C1s的纯化涉及多个步骤且耗时较长。这就解释了为何经常观察到这些亚组分的部分激活现象。在本报告中，我们提出了一种简化的纯化程序，采用批处理方法和快速蛋白质色谱法，避免了pH值的变化。该方法获得C1r和C1s的产率分别为35%和60%。此外，本研究提供了一种简单而灵敏的测试方法，以评估C1r和C1s相对于其他C1亚组分的功能纯度。
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