Dmitriev G A
Zh Mikrobiol Epidemiol Immunobiol. 1987 Dec(12):23-8.
The complex study of phosphohydrolases (Mg2+ATPase, Na+,K+ATPase) and adenylate cyclase in gonococci, both in vivo and in vitro, and in phagocytes in gonococcal infection has been carried out with the use of ultracytochemical techniques. The enzymatic activity in the cellular structures of micro- and macroorganisms under study has been visualized. The prospects of the ultracytochemical approach to the study of the enzymes of nucleotide metabolism, their role in the life of eu- and prokaryotes, as well as the ways for affecting the enzymatic systems of cells are discussed.
利用超细胞化学技术,对淋球菌体内外以及淋球菌感染时吞噬细胞中的磷酸水解酶(Mg2+ATP酶、Na+,K+ATP酶)和腺苷酸环化酶进行了复杂的研究。已观察到所研究的微生物细胞结构中的酶活性。讨论了超细胞化学方法在核苷酸代谢酶研究、它们在真核生物和原核生物生命中的作用以及影响细胞酶系统途径方面的前景。