Ma Min, Zhai Chun-Xia, Sun Cai-Xia
1 Department of Emergency, Laiwu City People's Hospital , Laiwu, P.R. China .
2 Department of Gastrointestinal, Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University , Changchun, P.R. China .
Genet Test Mol Biomarkers. 2017 Apr;21(4):206-212. doi: 10.1089/gtmb.2016.0243. Epub 2017 Mar 23.
This case-control study explored correlations between LP-PLA2 gene polymorphisms (A379V, V279F, and R92H) and susceptibility and severity of acute pancreatitis (AP) in a Chinese population.
From October 2013 to October 2015, 94 AP patients were chosen as the case group. According to the Acute Physiology and Chronic Health Evaluation (APACHE) II score standard, AP patients were divided into a mild AP (MAP) group (n = 46) and severe AP (SAP) group (n = 48). The 48 SAP patients were further divided into an SAP with multiple organ dysfunction syndrome (MODS) group (n = 42) and SAP without MODS group (n = 6). Meanwhile, 96 healthy subjects who received physical examinations at the study hospitals were selected as the control group. Serum lipoprotein-associated phospholipase A2 (LP-PLA2) levels were detected by an enzyme-linked immunosorbent assay (ELISA). The A379V (s1051931), V279F (rs16874954), and R92H (rs13989) polymorphisms of the LP-PLA2 gene were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
There were significant differences in the frequencies of the LP-PLA2 gene polymorphisms between the AP group and the control group. The distribution of V279F-AA+AC genotype and R92H-AA+AG genotype in the AP group was higher than that in the control group, whereas the SAP group and SAP with MODS group distributions were higher than those in the MAP group and SAP without MODS group (both p < 0.05). G-C-A, G-A-G, and G-C-G haploids formed by A379V, V279F, and R92H may be associated with AP susceptibility. LP-PLA2 gene polymorphisms could affect serum LP-PLA2 level, whereas the V279F-A allele gene, the R92H-A allele gene, serum LP-PLA2 level, and serum amylase may be independent risk factors for AP (all p < 0.05).
These results demonstrated that the LP-PLA2 gene polymorphisms, V279F and R92H, may be associated with susceptibility to and severity of AP.
本病例对照研究探讨脂蛋白相关磷脂酶A2（LP-PLA2）基因多态性（A379V、V279F和R92H）与中国人群急性胰腺炎（AP）易感性及严重程度之间的相关性。
2013年10月至2015年10月，选取94例AP患者作为病例组。根据急性生理与慢性健康状况评分系统（APACHE）Ⅱ评分标准，将AP患者分为轻症急性胰腺炎（MAP）组（n = 46）和重症急性胰腺炎（SAP）组（n = 48）。48例SAP患者进一步分为伴多器官功能障碍综合征（MODS）的SAP组（n = 42）和不伴MODS的SAP组（n = 6）。同时，选取在研究医院进行体检的96例健康受试者作为对照组。采用酶联免疫吸附测定（ELISA）法检测血清脂蛋白相关磷脂酶A2（LP-PLA2）水平。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）法检测LP-PLA2基因的A379V（s1051931）、V279F（rs16874954）和R92H（rs13989）多态性。
AP组与对照组之间LP-PLA2基因多态性频率存在显著差异。AP组中V279F-AA + AC基因型和R92H-AA + AG基因型的分布高于对照组，而SAP组和伴MODS的SAP组的分布高于MAP组和不伴MODS的SAP组（均p < 0.05）。由A379V、V279F和R92H形成的G-C-A、G-A-G和G-C-G单倍型可能与AP易感性相关。LP-PLA2基因多态性可影响血清LP-PLA2水平，而V279F-A等位基因、R92H-A等位基因、血清LP-PLA2水平和血清淀粉酶可能是AP的独立危险因素（均p < 0.05）。
这些结果表明，LP-PLA2基因多态性V279F和R92H可能与AP的易感性和严重程度相关。
