Loeber G, Dörries K
Institut für Virologie und Immunobiologie, Universität Würzburg, Federal Republic of Germany.
J Virol. 1988 May;62(5):1730-5. doi: 10.1128/JVI.62.5.1730-1735.1988.
Variants of JC virus (JCV) strain GS were isolated directly from the central nervous system (variant GS/B) and the kidney (variant GS/K) of a patient with progressive multifocal leukoencephalopathy and were cloned and sequenced. The genomes of the isolates were shown to be nearly identical in the nucleotide sequences of their protein-coding regions, suggesting that both had originated from a single infecting JCV genome. In contrast, the arrangement of the putative elements of transcriptional control revealed considerable differences. The tandemly repeated elements found twice within the enhancer region of JCV GS/B variant were not present in the GS/K variant. The missing elements were replaced by DNA segments containing simian virus 40 and adenovirus E1A core enhancer elements. These differences in the organ-specific GS variants suggest that rearrangements within elements of transcriptional control might be involved in altering the virus-cell interaction in the course of a JCV infection.
从一名进行性多灶性白质脑病患者的中枢神经系统（GS/B变体）和肾脏（GS/K变体）中直接分离出JC病毒（JCV）毒株GS的变体，并进行克隆和测序。结果显示，分离株的基因组在其蛋白质编码区的核苷酸序列上几乎相同，这表明两者都起源于单个感染性JCV基因组。相比之下，转录控制假定元件的排列显示出相当大的差异。在JCV GS/B变体增强子区域中发现两次的串联重复元件在GS/K变体中不存在。缺失的元件被包含猴病毒40和腺病毒E1A核心增强子元件的DNA片段所取代。器官特异性GS变体中的这些差异表明，转录控制元件内的重排可能参与了JCV感染过程中病毒与细胞相互作用的改变。
