Borish L, Audera C, Rosenbaum R, Rocklin R E
Allergy Division, New England Medical Center, Boston, Massachusetts 02111.
Cell Immunol. 1988 May;113(2):320-8. doi: 10.1016/0008-8749(88)90030-5.
The lymphokine leukocyte inhibitory factor (LIF) has previously been documented to enhance several neutrophil (PMN) functions, including stimulated chemotaxis and superoxide generation, phagocytosis and adherence of opsonized targets, and antibody-dependent cellular cytotoxicity. The present studies were designed to investigate the effects of LIF on PMN function mediated by the complement components C3b and C3bi. LIF induced a dose-dependent increase in superoxide production generated by opsonized zymosan (up to 97.1 +/- 31.4% at 16 U LIF/ml; P less than 0.01). While neither control nor LIF-treated PMN were capable of inducing phagocytosis of either C3b- or C3bi-opsonized sheep erythrocytes (E) directly, exposure to LIF caused a significant (P less than 0.05) increase in their adherence to E (137.4 and 59.4%, respectively). Specificity for complement receptor function was confirmed by the ability of anti-CR1 antibody to block adherence of LIF-treated PMN to EAC3b (77.0% inhibition) and anti-CR3 antibody to block adherence to EAC3bi (70.2% inhibition). Increased C3b and C3bi function may have been due, at least in part, to increased expression of their respective surface membrane receptors. Thus, using indirect immunofluorescence, LIF induced a 38.2% increase in fluorescence of the anti-CR1 antibody and a 96.1% increase in anti-CR3 binding. These studies describe an additional mechanism through which LIF may have an important pro-inflammatory role in vivo.
淋巴因子白细胞抑制因子（LIF）此前已有文献记载可增强多种中性粒细胞（PMN）功能，包括刺激趋化性和超氧化物生成、吞噬作用以及调理素化靶标的黏附，还有抗体依赖性细胞毒性。本研究旨在探讨LIF对由补体成分C3b和C3bi介导的PMN功能的影响。LIF诱导经调理的酵母聚糖产生的超氧化物产量呈剂量依赖性增加（在16 U LIF/ml时高达97.1±31.4%；P<0.01）。虽然对照和LIF处理的PMN均不能直接诱导吞噬C3b或C3bi调理的绵羊红细胞（E），但暴露于LIF会使其与E的黏附显著增加（P<0.05）（分别为137.4%和59.4%）。抗CR1抗体阻断LIF处理的PMN与EAC3b黏附的能力（77.0%抑制率）以及抗CR3抗体阻断与EAC3bi黏附的能力（70.2%抑制率）证实了补体受体功能的特异性。C3b和C3bi功能的增强可能至少部分归因于其各自表面膜受体表达的增加。因此，使用间接免疫荧光法，LIF使抗CR1抗体的荧光增加了38.2%，抗CRi3结合增加了96.1%。这些研究描述了LIF在体内可能具有重要促炎作用的另一种机制。
