Pataki Emese, Sipiczki Matthias, Miklos Ida
Department of Genetics and Applied Microbiology, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1, Debrecen, 4032, Hungary.
Curr Microbiol. 2017 Jun;74(6):710-717. doi: 10.1007/s00284-017-1227-9. Epub 2017 Mar 24.
Environmental glucose is an important regulator of biological processes, as it can launch different cell processes depending on its concentration. Thus, low glucose concentration can induce entry into quiescence, which ensures long-term viability for the cells or in other cases mycelial growth in the dimorphic species, which, in turn, provides the cells with fresh nutrients. Several genes, such as the genes of cAMP cascade, are involved in glucose sensing and response. Since this signal transduction pathway seemed to be an evolutionarily conserved process, we assumed that its genes were also conserved and preserved their functional homology. To obtain evidence, Schizosaccharomyces pombe rsv1 and its orthologous genes were investigated using in silico and experimental approaches. Our results supported that the Rsv1 zinc-finger transcription factors of Schizosaccharomyces japonicus and Schizosaccharomyces octosporus and the Candida albicans cas5p were really functional homologues of the S. pombe Rsv1. Namely, the homologous proteins were able to restore mutant phenotype of the S. pombe rsv1-deleted cells. Bioinformatic anaysis revealed that the most conserved parts of the proteins always contained the C2H2 domains and the complementation abilities of the counterpart genes were not uniform regarding the investigated features, which can be in connection with the conserved regions outside C2H2.
环境葡萄糖是生物过程的重要调节因子，因为它可以根据其浓度启动不同的细胞过程。因此，低葡萄糖浓度可诱导细胞进入静止状态，这确保了细胞的长期生存能力，或者在其他情况下，确保了二态性物种中的菌丝体生长，进而为细胞提供新鲜营养。一些基因，如环磷酸腺苷（cAMP）级联反应的基因，参与葡萄糖感知和反应。由于这种信号转导途径似乎是一个进化上保守的过程，我们推测其基因也是保守的，并保留了它们的功能同源性。为了获得证据，我们使用计算机模拟和实验方法对粟酒裂殖酵母rsv1及其直系同源基因进行了研究。我们的结果支持了日本裂殖酵母、八孢裂殖酵母的Rsv1锌指转录因子以及白色念珠菌的cas5p确实是粟酒裂殖酵母Rsv1的功能同源物。也就是说，同源蛋白能够恢复粟酒裂殖酵母rsv1缺失细胞中的突变表型。生物信息学分析表明，这些蛋白质中最保守的部分总是包含C2H2结构域，并且对应基因的互补能力在研究的特征方面并不一致，这可能与C2H2以外的保守区域有关。
