Gerisch Günther
Max-Planck-Institut für Biologie (Abt. Weidel) Tübingen, Deutschland.
Zoologisches Institut der Universität, 78 Freiburg i. Br.
Wilhelm Roux Arch Entwickl Mech Org. 1962 Nov;153(6):603-620. doi: 10.1007/BF00576755.
The morphogenetic capacities of cells inDictyostelium discoideum, as well as of groups (agglutinates) of these cells are changed in a constant time course after food consumption has been stopped.Two questions were posed: 1. When is the capacity of aggregation at its maximum level in a culture of single suspended cells. 2. At what time do agglutinates answer an external stimulus immediately by establishing polarity.The maximum of aggregation capacity lies 8-10 hours after complete food exhaustion. This functional stage is cytologically characterized by accumulation of metachromatic granules in the cells [seeBONNER et al. (1955)]. - The polarization capacity of agglutinates depends primarily on the functional age of the cells (this means that the age of the cells is related to the time of complete food exhaustion), and secondly it depends on the age of the agglutinates. In the most favorable case the maximum response is reached 12 hours after complete food exhaustion.A difference of about 2 hours lies between the beginning of aggregation and polarization. This difference in time could explain the centerless aggregation patterns of a well synchronized cell population as well as the appearance of primary aggregation centers in non-synchronized cultures.
在停止进食后，盘基网柄菌细胞以及这些细胞群体（凝集物）的形态发生能力会在固定的时间进程中发生变化。提出了两个问题：1. 在单个悬浮细胞培养物中，聚集能力何时达到最高水平。2. 凝集物在何时通过建立极性立即对外部刺激做出反应。聚集能力的最大值出现在食物完全耗尽后的8 - 10小时。这个功能阶段在细胞学上的特征是细胞中出现异染粒的积累[见邦纳等人（1955年）的研究]。——凝集物的极化能力主要取决于细胞的功能年龄（这意味着细胞的年龄与食物完全耗尽的时间有关），其次取决于凝集物的年龄。在最有利的情况下，在食物完全耗尽后12小时达到最大反应。聚集开始和极化之间相差约2小时。这个时间差可以解释同步良好的细胞群体的无中心聚集模式以及非同步培养物中初级聚集中心的出现。
