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海胆U7小核RNA的功能分析

Functional analysis of the sea urchin U7 small nuclear RNA.

作者信息

Gilmartin G M, Schaufele F, Schaffner G, Birnstiel M L

机构信息

Institut für Molekularbiologie II, Universität Zürich, Switzerland.

出版信息

Mol Cell Biol. 1988 Mar;8(3):1076-84. doi: 10.1128/mcb.8.3.1076-1084.1988.

DOI:10.1128/mcb.8.3.1076-1084.1988
PMID:2835659
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363250/
Abstract

U7 small nuclear RNA (snRNA) is an essential component of the RNA-processing machinery which generates the 3' end of mature histone mRNA in the sea urchin. The U7 small nuclear ribonucleoprotein particle (snRNP) is classified as a member of the Sm-type U snRNP family by virtue of its recognition by both anti-trimethylguanosine and anti-Sm antibodies. We analyzed the function-structure relationship of the U7 snRNP by mutagenesis experiments. These suggested that the U7 snRNP of the sea urchin is composed of three important domains. The first domain encompasses the 5'-terminal sequences, up to about nucleotides 7, which are accessible to micrococcal nuclease, while the remainder of the RNA is highly protected and hence presumably bound by proteins. This region contains the sequence complementarities between the U7 snRNA and the histone pre-mRNA which have previously been shown to be required for 3' processing (F. Schaufele, G. M. Gilmartin, W. Bannwarth, and M. L. Birnstiel, Nature [London] 323:777-781, 1986). Nucleotides 9 to 20 constitute a second domain which includes sequences for Sm protein binding. The complementarities between the U7 snRNA sequences in this region and the terminal palindrome of the histone mRNA appear to be fortuitous and play only a secondary, if any, role in 3' processing. The third domain is composed of the terminal palindrome of U7 snRNA, the secondary structure of which must be maintained for the U7 snRNP to function, but its sequence can be drastically altered without any observable effect on snRNP assembly or 3' processing.

摘要

U7小核RNA（snRNA）是RNA加工机制的重要组成部分，该机制负责生成海胆中成熟组蛋白mRNA的3'末端。U7小核糖核蛋白颗粒（snRNP）因其能被抗三甲基鸟苷抗体和抗Sm抗体识别，而被归类为Sm型U snRNP家族的成员。我们通过诱变实验分析了U7 snRNP的功能-结构关系。这些实验表明，海胆的U7 snRNP由三个重要结构域组成。第一个结构域包含5'末端序列，直至约第7个核苷酸，这些序列可被微球菌核酸酶作用，而RNA的其余部分受到高度保护，因此推测与蛋白质结合。该区域包含U7 snRNA与组蛋白前体mRNA之间的序列互补性，先前已证明这是3'加工所必需的（F. Schaufele、G.M. Gilmartin、W. Bannwarth和M.L. Birnstiel，《自然》[伦敦]323:777 - 781，1986年）。第9至20个核苷酸构成第二个结构域，其中包括Sm蛋白结合序列。该区域U7 snRNA序列与组蛋白mRNA末端回文序列之间的互补性似乎是偶然的，在3'加工中仅起次要作用（如果有作用的话）。第三个结构域由U7 snRNA的末端回文序列组成，其二级结构对于U7 snRNP发挥功能必须得以维持，但其序列可被大幅改变而对snRNP组装或3'加工没有任何可观察到的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc50/363250/6c8a858dd2c7/molcellb00063-0081-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc50/363250/66842af12270/molcellb00063-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc50/363250/8c2d9618daa0/molcellb00063-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc50/363250/4d61c97a2698/molcellb00063-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc50/363250/d79bf2b6bd96/molcellb00063-0080-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc50/363250/581379170299/molcellb00063-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc50/363250/6c8a858dd2c7/molcellb00063-0081-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc50/363250/66842af12270/molcellb00063-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc50/363250/8c2d9618daa0/molcellb00063-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc50/363250/4d61c97a2698/molcellb00063-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc50/363250/d79bf2b6bd96/molcellb00063-0080-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc50/363250/581379170299/molcellb00063-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc50/363250/6c8a858dd2c7/molcellb00063-0081-b.jpg

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