手部移植受者排斥反应期间外周血中供体来源微颗粒的检测
Detection of Donor-Derived Microparticles in the Peripheral Blood of a Hand Transplant Recipient During Rejection.
作者信息
Kim Joseph Y, Kelesidis Theodoros, Yang Otto O
机构信息
Division of Infectious Diseases, Department of Medicine, Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA.
Division of Infectious Diseases, Department of Medicine, Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA.; Department of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, CA.
出版信息
Transplant Direct. 2017 Feb 7;3(3):e131. doi: 10.1097/TXD.0000000000000646. eCollection 2017 Mar.
BACKGROUND
Microparticles (MPs) are released from the plasma membrane of activated or dying cells and bear surface molecules from those cells. We examined whether donor-derived MPs in the peripheral blood of the recipient could serve as a marker of tissue damage due to rejection of a transplanted hand.
METHODS
Platelet-free plasma from the recipient of the transplanted hand was analyzed for MPs bearing the donor-specific HLA molecule A*02 using flow cytometry. Rejection status of the transplanted hand was monitored by histopathology of skin punch biopsies.
RESULTS
Donor-specific MPs expressing HLA A*02 were quantifiable in the peripheral blood of the recipient. Levels of these MPs increased with worsening rejection of the transplanted hand.
CONCLUSIONS
These findings demonstrate the ability to detect donor specific MPs through staining of graft cell-specific HLA and promote further investigation into the potential utility of flow cytometry for donor-derived MPs as a noninvasive tool to assess rejection in solid organ transplantation patients.
背景
微粒（MPs）从活化或濒死细胞的质膜释放，并带有来自这些细胞的表面分子。我们研究了受者外周血中供体来源的MPs是否可作为移植手排斥所致组织损伤的标志物。
方法
使用流式细胞术分析移植手受者的无血小板血浆中携带供体特异性HLA分子A*02的MPs。通过皮肤打孔活检的组织病理学监测移植手的排斥状态。
结果
在受者外周血中可定量检测到表达HLA A*02的供体特异性MPs。随着移植手排斥反应的加重，这些MPs的水平升高。
结论
这些发现证明了通过对移植细胞特异性HLA进行染色来检测供体特异性MPs的能力，并促进了对将流式细胞术用于供体来源MPs作为评估实体器官移植患者排斥反应的非侵入性工具的潜在效用的进一步研究。
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