通过自组织人类胚胎干细胞培养生成三维视网膜组织。

Generation of a Three-Dimensional Retinal Tissue from Self-Organizing Human ESC Culture.

作者信息

Kuwahara Atsushi, Nakano Tokushige, Eiraku Mototsugu

机构信息

In Vitro Histogenesis team, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan.

Regenerative and Cellular Medicine Office, Sumitomo Dainippon Pharma Co., Ltd., Chuo Kobe, 650-0047, Japan.

出版信息

Methods Mol Biol. 2017;1597:17-29. doi: 10.1007/978-1-4939-6949-4_2.

DOI:10.1007/978-1-4939-6949-4_2

PMID:28361307

Abstract

A three-dimensional (3D) tissue generated in vitro is a promising source to study developmental biology and regenerative medicine. In the last decade, Yoshiki Sasai's group have developed a 3D stem cell culture technique known as SFEBq and demonstrated that embryonic stem cells (ESCs) have an ability to self-organize stratified neural tissue including 3D-retina. Furthermore, we have reported that ESC-derived retinal tissue can form an optic cup and a ciliary margin, which are unique structures in the developing retina. In this review, we focus on self-organizing culture technique to generate 3D-retina from human ESCs.

摘要

体外生成的三维（3D）组织是研究发育生物学和再生医学的一个有前景的来源。在过去十年中，笹井芳树的团队开发了一种称为SFEBq的3D干细胞培养技术，并证明胚胎干细胞（ESC）具有自我组织形成分层神经组织（包括3D视网膜）的能力。此外，我们已经报道，ESC来源的视网膜组织可以形成视杯和睫状缘，这是发育中的视网膜中的独特结构。在这篇综述中，我们重点关注从人类ESC生成3D视网膜的自我组织培养技术。