College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China; Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, China.
School of Physics and Electronic Engineering, Xinyang Normal University, Xinyang 464000, China; Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, China.
Biosens Bioelectron. 2017 Aug 15;94:616-625. doi: 10.1016/j.bios.2017.03.058. Epub 2017 Mar 30.
An ultrasensitive sandwich-type electrochemical biosensor for DNA detection is developed based on spherical silicon dioxide/molybdenum selenide (SiO@MoSe) and graphene oxide-gold nanoparticles (GO-AuNPs) hybrids as carrier triggered Hybridization Chain Reaction (HCR) coupling with multi-signal amplification. The proposed sensoring assay utilizes a spherical SiO@MoSe/AuNPs as sensing platform and GO-AuNPs hybrids as carriers to supply vast binding sites. HO+HQ system is used for DNA detection and HCR as the signal and selectivity enhancer. The sensor is designed in sandwich type to increase the specificity. As a result, the present biosensor exhibits a good dynamic range from 0.1fM to 100pM with a low detection limit of 0.068fM (S/N=3). This work shows a considerable potential for quantitative detection of DNA in early clinical diagnostics.
基于球形二氧化硅/二硒化钼（SiO@MoSe）和氧化石墨烯-金纳米粒子（GO-AuNPs）杂化物作为载体引发杂交链式反应（HCR）并与多信号放大耦合，开发了一种用于 DNA 检测的超灵敏夹心型电化学生物传感器。所提出的传感分析利用球形 SiO@MoSe/AuNPs 作为传感平台和 GO-AuNPs 杂化物作为载体提供大量结合位点。HO+HQ 体系用于 DNA 检测，HCR 作为信号和选择性增强剂。传感器采用三明治结构设计，以提高特异性。结果，该生物传感器在 0.1fM 至 100pM 的宽动态范围内表现出良好的线性，检测限低至 0.068fM（S/N=3）。这项工作为临床早期诊断中 DNA 的定量检测展示了相当大的潜力。
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