• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

抑制酪蛋白激酶2可阻断小鼠早期胚胎有丝分裂中的G/M转换，但不影响卵母细胞减数分裂。

Inhibition of casein kinase 2 blocks G/M transition in early embryo mitosis but not in oocyte meiosis in mouse.

作者信息

Lin Fei, Cao Shi-Bing, Ma Xue-Shan, Sun Hai-Xiang

机构信息

Center for Reproductive Medicine, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China.

State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.

出版信息

J Reprod Dev. 2017 Jun 21;63(3):319-324. doi: 10.1262/jrd.2016-064. Epub 2017 Mar 26.

DOI:10.1262/jrd.2016-064
PMID:28367932
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5481635/
Abstract

Casein kinase 2 (CK2) is a highly conserved, ubiquitously expressed serine/threonine protein kinase with hundreds of substrates. The role of CK2 in the G/M transition of oocytes, zygotes, and 2-cell embryos was studied in mouse by enzyme activity inhibition using the specific inhibitor 4, 5, 6, 7-tetrabromobenzotriazole (TBB). Zygotes and 2-cell embryos were arrested at G phase by TBB treatment, and DNA damage was increased in the female pronucleus of arrested zygotes. Further developmental ability of arrested zygotes was reduced, but that of arrested 2-cell embryos was not affected after releasing from inhibition. By contrast, the G/M transition in oocytes was not affected by TBB. These results indicate that CK2 activity is essential for mitotic G/M transition in early embryos but not for meiotic G/M transition in oocytes.

摘要

酪蛋白激酶2（CK2）是一种高度保守、广泛表达的丝氨酸/苏氨酸蛋白激酶，有数百种底物。在小鼠中，通过使用特异性抑制剂4,5,6,7-四溴苯并三唑（TBB）抑制酶活性，研究了CK2在卵母细胞、受精卵和2-细胞胚胎的G/M期转换中的作用。TBB处理使受精卵和2-细胞胚胎停滞在G期，停滞受精卵的雌性原核中的DNA损伤增加。停滞受精卵的进一步发育能力降低，但从抑制中释放后，停滞2-细胞胚胎的发育能力不受影响。相比之下，TBB不影响卵母细胞中的G/M期转换。这些结果表明，CK2活性对于早期胚胎的有丝分裂G/M期转换至关重要，但对于卵母细胞的减数分裂G/M期转换并非必需。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17fa/5481635/411e2fabc72f/jrd-63-319-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17fa/5481635/4d1866c47ca3/jrd-63-319-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17fa/5481635/221483e0881b/jrd-63-319-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17fa/5481635/4767592c7a4a/jrd-63-319-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17fa/5481635/b04d4d632912/jrd-63-319-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17fa/5481635/1290ebddc792/jrd-63-319-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17fa/5481635/411e2fabc72f/jrd-63-319-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17fa/5481635/4d1866c47ca3/jrd-63-319-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17fa/5481635/221483e0881b/jrd-63-319-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17fa/5481635/4767592c7a4a/jrd-63-319-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17fa/5481635/b04d4d632912/jrd-63-319-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17fa/5481635/1290ebddc792/jrd-63-319-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17fa/5481635/411e2fabc72f/jrd-63-319-g006.jpg

相似文献

1
Inhibition of casein kinase 2 blocks G/M transition in early embryo mitosis but not in oocyte meiosis in mouse.抑制酪蛋白激酶2可阻断小鼠早期胚胎有丝分裂中的G/M转换，但不影响卵母细胞减数分裂。
J Reprod Dev. 2017 Jun 21;63(3):319-324. doi: 10.1262/jrd.2016-064. Epub 2017 Mar 26.
2
Regulation of the G2/M transition in oocytes of xenopus tropicalis.热带爪蟾卵母细胞中G2/M期转换的调控
Dev Biol. 2003 Aug 15;260(2):438-48. doi: 10.1016/s0012-1606(03)00259-8.
3
Inhibition of casein kinase II reduces TGFβ induced fibroblast activation and ameliorates experimental fibrosis.抑制酪蛋白激酶 2 可减少 TGFβ 诱导的成纤维细胞活化并改善实验性纤维化。
Ann Rheum Dis. 2015 May;74(5):936-43. doi: 10.1136/annrheumdis-2013-204256. Epub 2014 Jan 15.
4
CenpH regulates meiotic G2/M transition by modulating the APC/CCdh1-cyclin B1 pathway in oocytes.CenpH通过调节卵母细胞中的APC/CCdh1-细胞周期蛋白B1途径来调控减数分裂G2/M期转换。
Development. 2017 Jan 15;144(2):305-312. doi: 10.1242/dev.141135. Epub 2016 Dec 19.
5
Zygote arrest 1 (Zar1) is a novel maternal-effect gene critical for the oocyte-to-embryo transition.合子阻滞1（Zar1）是一种对卵母细胞向胚胎转变至关重要的新型母源效应基因。
Nat Genet. 2003 Feb;33(2):187-91. doi: 10.1038/ng1079. Epub 2003 Jan 21.
6
Aurora-A is a critical regulator of microtubule assembly and nuclear activity in mouse oocytes, fertilized eggs, and early embryos.极光激酶A是小鼠卵母细胞、受精卵和早期胚胎中微管组装和核活性的关键调节因子。
Biol Reprod. 2004 May;70(5):1392-9. doi: 10.1095/biolreprod.103.025155. Epub 2003 Dec 26.
7
Overexpression of CDC14B causes mitotic arrest and inhibits zygotic genome activation in mouse preimplantation embryos.CDC14B 的过表达导致小鼠胚胎的有丝分裂停滞并抑制合子基因组激活。
Cell Cycle. 2009 Dec;8(23):3904-13. doi: 10.4161/cc.8.23.10074. Epub 2009 Dec 14.
8
Selectivity of 4,5,6,7-tetrabromobenzotriazole, an ATP site-directed inhibitor of protein kinase CK2 ('casein kinase-2').4,5,6,7-四溴苯并三唑（一种蛋白激酶CK2（酪蛋白激酶-2）的ATP位点定向抑制剂）的选择性
FEBS Lett. 2001 May 4;496(1):44-8. doi: 10.1016/s0014-5793(01)02404-8.
9
Involvement of insulin in early development of mouse one-cell stage embryos.胰岛素在小鼠单细胞期胚胎早期发育中的作用。
Sci China C Life Sci. 2008 Sep;51(9):767-73. doi: 10.1007/s11427-008-0113-x. Epub 2008 Aug 24.
10
CK2 inhibition induces apoptosis via the ER stress response.CK2 抑制通过内质网应激反应诱导细胞凋亡。
Cell Signal. 2011 Jan;23(1):145-51. doi: 10.1016/j.cellsig.2010.08.014. Epub 2010 Aug 31.

引用本文的文献

1
Casein kinase 2 complex: a central regulator of multiple pathobiological signaling pathways in .酪蛋白激酶 2 复合物：在 中多种病理生物学信号通路的核心调节因子。
mBio. 2024 Feb 14;15(2):e0327523. doi: 10.1128/mbio.03275-23. Epub 2024 Jan 9.
2
Simultaneous CK2/TNIK/DYRK1 inhibition by 108600 suppresses triple negative breast cancer stem cells and chemotherapy-resistant disease.108600 同时抑制 CK2/TNIK/DYRK1，抑制三阴性乳腺癌干细胞和化疗耐药性疾病。
Nat Commun. 2021 Aug 3;12(1):4671. doi: 10.1038/s41467-021-24878-z.
3
Deoxypodophyllotoxin Inhibits Cell Growth and Induces Apoptosis by Blocking EGFR and MET in Gefitinib-Resistant Non-Small Cell Lung Cancer.

本文引用的文献

1
Inhibition of CK2 enhances UV-triggered apoptotic cell death in lung cancer cell lines.抑制 CK2 可增强肺癌细胞系中 UV 触发的细胞凋亡。
Oncol Rep. 2013 Jul;30(1):377-84. doi: 10.3892/or.2013.2407. Epub 2013 Apr 18.
2
Activation of protein kinase CK2 attenuates FOXO3a functioning in a PML-dependent manner: implications in human prostate cancer.蛋白激酶 CK2 的激活以依赖于 PML 的方式减弱 FOXO3a 的功能：在人前列腺癌中的意义。
Cell Death Dis. 2013 Mar 14;4(3):e543. doi: 10.1038/cddis.2013.63.
3
Oocytes progress beyond prophase in the presence of DNA damage.
脱氧鬼臼毒素通过阻断 EGFR 和 MET 抑制吉非替尼耐药非小细胞肺癌细胞生长并诱导细胞凋亡。
J Microbiol Biotechnol. 2021 Apr 28;31(4):559-569. doi: 10.4014/jmb.2101.01029.
4
Casein kinase 2 modulates the spindle assembly checkpoint to orchestrate porcine oocyte meiotic progression.酪蛋白激酶2调节纺锤体组装检查点以协调猪卵母细胞减数分裂进程。
J Anim Sci Biotechnol. 2020 Apr 8;11:31. doi: 10.1186/s40104-020-00438-1. eCollection 2020.
卵母细胞在存在 DNA 损伤的情况下会进入前期后期。
Curr Biol. 2012 Jun 5;22(11):989-94. doi: 10.1016/j.cub.2012.03.063. Epub 2012 May 10.
4
Inhibition of Casein kinase-2 induces p53-dependent cell cycle arrest and sensitizes glioblastoma cells to tumor necrosis factor (TNFα)-induced apoptosis through SIRT1 inhibition.抑制酪蛋白激酶-2 通过抑制 SIRT1 诱导 p53 依赖性细胞周期停滞并增强胶质母细胞瘤细胞对肿瘤坏死因子 (TNFα) 诱导的细胞凋亡的敏感性。
Cell Death Dis. 2012 Feb 9;3(2):e271. doi: 10.1038/cddis.2012.10.
5
Design and synthesis of CK2 inhibitors.CK2 抑制剂的设计与合成。
Mol Cell Biochem. 2011 Oct;356(1-2):91-6. doi: 10.1007/s11010-011-0953-8. Epub 2011 Jul 13.
6
Protein kinase CK2--a key suppressor of apoptosis.蛋白激酶CK2——细胞凋亡的关键抑制因子。
Adv Enzyme Regul. 2008;48:179-87. doi: 10.1016/j.advenzreg.2008.04.002. Epub 2008 Apr 30.
7
The regulatory beta-subunit of protein kinase CK2 regulates cell-cycle progression at the onset of mitosis.蛋白激酶CK2的调节性β亚基在有丝分裂开始时调节细胞周期进程。
Oncogene. 2008 Aug 28;27(37):4986-97. doi: 10.1038/onc.2008.146. Epub 2008 May 12.
8
A role for CK2 upon interkinetic nuclear migration in the cell cycle of retinal progenitor cells.CK2在视网膜祖细胞细胞周期中的核内有丝分裂迁移过程中所起的作用。
Dev Neurobiol. 2008 Apr;68(5):620-31. doi: 10.1002/dneu.20613.
9
The alpha catalytic subunit of protein kinase CK2 is required for mouse embryonic development.蛋白激酶CK2的α催化亚基是小鼠胚胎发育所必需的。
Mol Cell Biol. 2008 Jan;28(1):131-9. doi: 10.1128/MCB.01119-07. Epub 2007 Oct 22.
10
A CK2-dependent mechanism for degradation of the PML tumor suppressor.一种依赖CK2的PML肿瘤抑制因子降解机制。
Cell. 2006 Jul 28;126(2):269-83. doi: 10.1016/j.cell.2006.05.041.